We provide the means for a fast and gentle isolation and flow sorting of regulatory T cells (Treg cells) as well as distinct Treg subsets. You have the possibility of optimized culture, activation, and expansion, as well as phenotyping and functional analysis of Treg cells.
Regulatory T cells (Tregs) are an important T cell subpopulation that maintains immunological homeostasis. Today, Treg-based therapy shows an enormous potential for treatment of autoimmune and auto-inflammatory disorders, acute and chronic infection, allergy, metabolic inflammation, transplantation, and cancer. Therefore, it is crucial to provide reliable and trustworthy research materials alongside an easily translatable workflow into the clinic. This webinar explores our innovative tools to support Treg research at every step.
Tregs represent 1–4% of all lymphocytes in secondary lymphoid organs (such as spleen and lymph node) of wild type mice. Spleen and lymph nodes must be dissociated into a single-cell suspension for many downstream applications. Dissociation can be accomplished with specific Tissue Dissociation Kits (e.g. Spleen Dissociation Kit, mouse) manually or in a fast and convenient way by using the automatic gentleMACS™ Dissociator.
We offer a broad range of products for the isolation of Treg cells according to your starting material and species; human, mouse, and non-human primate. MACS® MicroBeads and Isolation Kits are the best products for isolation of highly pure and viable distinct Treg subsets.
Isolation of human CD4+CD25+CD127dim/-Regulatory T Cell using MACS® Technology
Our MACSxpress® Treg Isolation Kit, human has been developed for the isolation of CD4+CD25+ Treg cells from up to 30 mL of freshly drawn anti-coagulated whole blood. Isolate pure Treg cells in only 30 minutes, without the requirement of density gradient centrifugation.
Tregs are fragile and sensitive to mechanical stress. Therefore, gentle isolation is a critical step for accurate downstream (functional and phenotypical) analyses and applications.
The MACSQuant® Tyto® Cell Sorter is revolutionizing cell sorting. Our patented microchip-based technology opens up new possibilities in basic research and medical applications with high-speed multiparameter cell sorting in the safety of a fully enclosed and sterile cartridge system, the MACSQuant Tyto Cartridge.
The MACSQuant Tyto Cell Sorter gives you the opportunity to sort different subtype of Tregs not only for your research purposes but also for cell and gene therapy application.
Human Treg sorting by MACSQuant® Tyto® CellSorter:
Activation, expansion, and stimulation of Treg cells can be very challenging. The combination of TexMACS™ Medium, MACS® Cytokines, and Treg expansion kits (human and mouse) are perfectly suited for in vitro culture and expansion of Treg cells.
TexMACS™ Medium is an optimized serum-free cell culture medium developed for the cultivation and expansion of human and mouse T cells and regulatory T cells.
The Treg Suppression Inspector has been developed for the in vitro functional characterization of human CD4+CD25+regulatory T cells.
Treg suppression assay using the Treg Suppression Inspector, human:
The Treg Detection Kits (mouse and human) are optimized antibody kits with pre-titrated antibodies that ensure a reliable flow cytometric analysis of Treg cells. Kits are ready-to-use cocktail, which conveniently reduces pipetting steps.
REAfinity™ Antibodies are recombinant antibodies that provide superior lot-to-lot consistency and purity compared to mouse or rat hybridoma-derived, monoclonal antibodies.
REAfinity™ Recombinant Antibody model:
Cesar Evaristo, Marco Vahldieck, Susanne Krauthaeuser, Nicole Jansen, Niklas Wilske, Anne Richter, and Christian Dose