Isolation and analysis of dendritic cells and their subsets

Discover this optimized workflow for isolating and characterizing human and mouse dendritic cells (DCs). It provides the ability to isolate and analyze CD11c+ DCs and their subsets. Plus, our PepTivator® Technology provides the peptide pools for antigen loading.

DCs are the sentinels of the immune system and are especially located at the interface between the body and external environment. Isolation of DCs from different tissues can be performed by using the combination of gentleMACS™ Octo Dissociator with Heaters, the appropriate tissue dissociation kit, and MACS® MicroBeads.

Flow analysis of DC subsets from enriched mouse CD11c+ DCs

CD11c+ DC isolation and subset analysis

Pan DCs can be isolated from dissociated tissues such as spleen, lung, or lamina propria using CD11c MicroBeads UltraPure. Enriched DCs show high viability and preserved epitopes enabling the detailed analysis of distinct subsets using MACS® antibodies.

Video
Isolation of CD11c+ dendritic cells from mouse spleen 

Watch how easy it is to manually separate DCs from mouse tissue using MACS Technology.

Poster
Human and mouse dendritic cell generation

Download this poster to find all DC surface markers and products you need for convenient isolation and analysis.

Efficient loading of DCs with antigens is key for antigen presentation to T cells. By using PepTivator® Peptide Pools, antigen loading has never been easier. There are several ways a dendritic cell can be loaded with an antigen. Once option is to add into culture peptides that will be phagocyted and presented on MHC molecules. 

Structure of Peptivator® Peptide Pools

PepTivator Peptide Pools for antigen loading

We offer a broad range of PepTivator peptide pools. This extensive panel of virus- or tumor-specific antigens consists of 15-mer peptides with 11-amino-acid overlaps, covering the complete sequence of the respective antigen.

Culturing DCs together with PepTivators leads to peptide phagocytosis and presentation on MHC molecules.

 

Characterization of DCs can be a challenging task. Using our REAfinity™ Recombinant Antibodies, flow cytometry analysis of human and mouse primary DC populations is easy and reproducible. Download the DC quick guide and browse through the broadest portfolio on DC generation, isolation, and analysis in the field.

Quick guide
The broadest portfolio for your dendritic cell research

Get acquainted with our protocols and antibody panels for the isolation and analysis of dendritic cells and their subsets.

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