Umbilical cord-derived mesenchymal stem cells (MSCs)

Maintaining and expanding your umbilical cord derived mesenchymal stem cells (MSCs) is of first priority for standardized and differentiation potential. No matter your experimental needs, our solutions include complete workflow service from tissue dissection to MSC isolation, expansion, differentiation and immunomodulation assays. With specialized dissociation kits involving automated dissociation of umbilical cord tissue, the potential for MSC expansion in our xeno- and serum-free medium provide the structural foundation and continued support you need for viable downstream applications.

Brochure
Workflow for human mesenchymal stem or stromal cells (MSCs)

Human umbilical cord tissue can be dissociated into a single-cell suspension by combining mechanical dissociation with enzymatic degradation of the extracellular adhesion proteins that maintain the tissue's structural integrity. Our Umbilical Cord Dissociation Kit, human and the gentleMACS Dissociators are the perfect combination for standardized and reliable dissociation of umbilical cord tissue.

Video
gentleMACS Dissociators: Start smart!

Get your research off to the right start. Rely on excellent sample preparation with the gentleMACS Dissociator family. Start your experiments off smart with standardized and reproducible tissue dissociation.

Our cell culture options provide reliable solutions for the maintenance and expansion of MSCs with preserved differentiation potential and immunomodulation ability.

MSCs cultured in StemMACS MSC Expansion Media XF show an increased expansion rate compared to other standard MSC culture methods

StemMACS™ MSC Expansion Media Kit XF, human

  • Robust expansion of human MSCs in xeno- and serum-free conditions
  • No substrate pre-coating of the culture vessels required
  • No additional supplement or growth factors or serum required
  • Upgrade to MACS GMP medium possible to facilitate clinical translation

 

The MSC Phenotyping Kit was developed for the standardized identification and phenotyping of cultured human MSCs by flow cytometry based on the defined ISCT standards.

Phenotyping of in vitro expanded CD271+ MSCs and PA-MSCs

MSC Phenotyping Kit

  • Phenotyping based on the expression of CD73, CD90, CD105 and exclusion of CD14, CD20, CD34, and CD45 markers
  • Anibodies for isotype controls and compensation of PerCP, PE, APC, and FITC channels are included

 

We provide a variety of media options that support the differentiation of MSCs into adipocytes, osteoblasts, and chondrocytes. The different StemMACS Differentiation Media are suitable for the analysis or quality control of the differentiation capacity of expanded MSCs, as well as in vitro studies focusing on the processes involved in MSC differentiation, including gene expression and protein profiling.

Adipocytes stained with Oil Red O after cultivation of MSCs for 21 days in StemMACS AdipoDiff Medium.
Osteoblasts differentiated from human MSCs after cultivation for ten days in StemMACS OsteoDiff Medium stained with NBT substrate.
Chondrocytes stained for aggrecan (red) and nuclei (blue) after cultivation of MSCs for 21 days in StemMACS ChondroDiff Medium.

The suppression potential of MSCs varies among in vitro expanded cells and shows donor-dependent differences. MSCs can be “licensed” by inflammatory cytokines such IFN-γ and TNF-α to become more immunosuppressive and show a more homogeneous phenotype in this regard. The MSC Suppression Inspector, human was developed for standardized characterization of the immunomodulatory function of MSCs by co-culture with CD4+CD25 or CD4+ responder T (Tresp) cells.

Evaluation of T cell-specific immunmodulatory function of  PA-MSCs or CD271+ MSCs with the MSC Suppression Inspector, human

MSC Suppression Inspector, human 

  • The kit contains Anti-Biotin MACSiBead Particles pre-loaded with biotinylated CD2, CD3, and CD28 antibodies for optimal T cell stimulation
  • Protocol includes Tresp isolation and stimulation, MSC-Tresp co-culture, and analysis of Tresp cell proliferation by 3H-thymidine incorporation, as well as carboxyfluorescein succinimidyl ester (CFSE) staining

 

Application note
Effective licensing of human mesenchymal stem cells

This application note describes the licensing of MSCs using IFN-γ and TNF-α and a procedure to analyze marker expression and the immunosuppressive characteristics of MSCs using the MSC Suppression Inspector, human.

Get acquainted with our protocols for your workflows in mesenchymal stem cell research.

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