Tumor tissue storage and dissociation

Tumor tissue makes for highly valuable sample material. Samples are treated with utter care to prevent, for instance, sample degeneration or unwanted cell activation. Two delicate steps are sample transportation or storage and tissue dissociation into single-cell suspensions for further analysis. Here, we show how these two workflow steps can be mastered using optimized solutions for human or mouse tumor tissue handling.

Tumor tissue samples are often transported to the lab, for example, from collaboration sites, or they are stored while other samples are being processed. Optimal storage conditions keep tumor cells viable and prevent unwanted cell activation to enable reliable downstream analysis.

Safe tissue storage for up to 48 hours

Preserved tumor cell viability after 48 h of tissue storage

The MACS® Tissue Storage Solution enables storage of tissue for up to 48 h without compromising viability or causing unwanted effects like cell activation or apoptosis.

Preserved TIL composition after up to 48 h

Preserved TIL composition of stored tumor samples

Flow cytometry analysis of tumor-infiltrating lymphocyte (TIL) populations from mouse tumors stored in MACS Tissue Storage Solution show no changes in TIL composition.

Gentle tumor dissociation is a prerequisite for efficient downstream cell separation, flow analysis, cell sorting, molecular analysis, and cell culture. Only viable single-cell suspensions with preserved cell surface epitopes will allow for reliable downstream results.

Using the gentleMACS™ Tissue Dissociators with the gentleMACS C Tubes and Tumor Dissociation Kits (human and mouse), mechanical and enzymatic dissociation are combined to enable gentle, automated tumor dissociation for standardized and reproducible results. 

Video
Dissociation of tumor tissue (mouse) with the gentleMACS Dissociator

Watch how the gentleMACS Dissociator can help speed up your workflow of dissociating tumor tissue.

Cell viability after tumor dissociation with the Tumor Dissociation Kit

Viable single-cell suspensions even from very small biopsy samples

Dissociation of fresh tumors with gentleMACS Technology in combination with optimized protocols results in viable single-cell suspensions even for small biopsy samples.

Efficient recovery of TILs from B16-F10 tumors

Efficient recovery of rare TIL populations

Gentle enzymatic dissociation with Tumor Dissociation Kits allows for recovery of rare key populations of TILs, which would otherwise remain embedded in the tumor tissue.

Effective dissociation of FFPE samples

Effective dissociation of FFPE samples

Human formalin-fixed paraffin-embedded (FFPE) carcinoma sections are effectively dissociated with the FFPE Tissue Dissociation Kit. High yields of single cells can be obtained while preserving important epitopes to identify carcinoma cells.

Application note
Enrichment of tumor cells from FFPE lung adenocarcinoma resection specimens increases sensitivity of mutation analysis

Learn how our FFPE workflow can increase the sensitivity of NGS mutation analysis.

Video
How to dissociate FFPE tissue in some easy steps

Using our gentleMACS Octo Dissociator with Heaters, FFPE tissue can be easily dissociated to get single-cell suspensions for molecular analysis. The protocol allows preservation of cytokeratin and vimentin epitopes which allow the subsequent separation of tumor and non-tumor cells from human carcinoma FFPE samples prior molecular analysis.

Get acquainted with our protocols for the storage and dissociation of mouse and human tumor tissue.

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