With this workflow, you can rely on fast and easy isolation of pure NK cells or NK cell subsets and superior NK cell expansion. Furthermore, it uses optimized flow cytometry reagents for phenotyping and functional analyses.
Isolation of untouched human NK cells from PBMCs by using the NK Cell Isolation Kit, an LS Column, and a MidiMACS™ Separator. CD56+ cells were isolated from human PBMCs using the REAlease® CD56 MicroBead Kit, MS Columns, and a MiniMACS™ Separator. Cells were fluorescently stained with CD56-PE and analyzed by flow cytometry using the MACSQuant® Analyzer X. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
If you are looking for a solution to isolate multiple NK cell subsets, REAlease® CD56 MicroBead Kit is the perfect solution for you.
Philipp Steinbrueck, Zhong Yu, Ursula Bissa, Jennifer Brieden, and Christian Dose
Ex vivo cultivation is an attractive option to increase NK cells in numbers; either to study their biology or to improve their anti-tumor potential. But we know how difficult it is, that’s why we have developed the new NK MACS Medium. A designed formulation that offers the perfect solution to expand and activate human NK cells from various sources, including peripheral blood mononuclear cells (PBMCs) or isolated NK cells. Its defined formulation enables expansion of NK cells that are fully functional, ready for any downstream application.
Reliable activation and expansion of NK cells is a clear requirement for effective downstream experiments. NK MACS® Medium is optimized for NK cell expansion and is the perfect solution to culture NK cells in vitro.
NK cells can be expanded using PBMCs as starting material or previous to cultivation NK cells can be isolated from whole blood, buffy coats or PBMCs. Using NK MACS® Medium results in 3 times more NK cell fold expansion compared to competitor medium or TexMACS (T cell medium).
Easily translate your research NK cell culture protocol into a clinical-grade protocol using the new NK MACS GMP Medium (Phenol Red), together with our MACS GMP Cytokines.
As the final step in many workflows, flow analysis plays an important part in many downstream assays during NK cell analysis. The following flow reagents can ensure that the data you generate on your flow cytometer is reliable and consistent:
Build your own NK cell flow panel with our Flow Panel Builder.
Kalpana Singh, John P. Veluchamy², María Delso-Vallejo, Nina Kok, Fenna Bohme, Ruth Seggewiss-Bernhardt⁴, Hans J. van der Vliet, Tanja D. de Gruijl, Volker Huppert, and Jan Spanholtz