Brain organoids derived from pluripotent stem cells (PSCs), either embryonic or induced PSCs, have emerged as novel model systems that can be used to investigate human brain development and disorders. Browse through our brain organoid dissociation options for single cell analysis or our cutting-edge solutions for whole organoid visualization.
To obtain viable and reliable brain organoids it is pivotal to use high quality PSC lines. StemMACS™ iPS-Brew XF, human and StemMACS™ PSC-Brew XF, human have been specifically designed to provide your cells with the best-quality nutrients to sustain robust growth and maintain a high pluripotent phenotype and differentiation potential.
Generation of PSC-derived brain organoids is achieved by timely addition of patterning factors. For this sensitive phase, precise active cytokine or small molecule amount is pivotal, both for success of the differentiation itself but also to ensure experimental reproducibility. MACS® Premium-Grade Cytokines come with specific biological activity for each lot so you can precisely apply cytokines according to their biological activity. In this way, you can generate reproducible cell culture conditions and aim for natural cytokine concentrations while avoiding possibly harmful oversaturation, artificial experimental results, and at the same time reduce costs.
To meet experimental needs and read-out, dissociation of PSC-derived organoids into single cells might be necessary. These sensitive structures require specific reagents in order to obtain cells that are still viable and usable. Gently dissociate mature brain organoids and obtain highly viable cell populations for further adherent culture, separation, analysis by flow cytometry, or immunofluorescence staining.
With the Neural Tissue Dissociation Kit, a gentle two-step enzymatic dissociation procedure, you can obtain high numbers of viable cells in less than an hour. Preserve key epitopes by choosing the Neural Tissue Dissociation Kit (P) with papain or the Neural Tissue Dissociation Kit (T) with trypsin. After dissociation, cells can be maintained in culture with MACS Neuro Medium supplemented by MACS NeuroBrew®-21.
Flow cytometry is a quick, qualitative and quantitative analysis method that can be applied after dissociation to confirm the presence of cells of interest or analyze the expression of specific markers.
Brain organoids are complex structures and can be analyzed by evaluating many different markers. Browse through our large portfolio of antibodies for flow cytometry that provide superior lot-to-lot consistency and purity compared to mouse or rat hybridoma-derived, monoclonal antibodies. Our REAffinity antibodies have been recombinantly engineered to be highly specific without the need of blocking the Fc receptor.
Suitable flow antibodies for brain organoids
|Sox2 Antibody, anti-human/mouse, REAfinity||Stem cells|
|PAX-6 Antibody, anti-human, REAfinity||Stem cells|
|CD117 Antibody, anti-human, REAfinity (c-kit)||Stem cells|
|CD44 Antibody, anti-human, REAfinity||Stem cells|
|CD271 (LNGFR) Antibody, anti-human, REAfinity||Stem cells|
|CD24 Antibody, anti-human, REAfinity||Stem cells|
|CD29 Antibody, anti-human, REAfinity||Stem cells|
|GFAP Antibody, anti-human/mouse/rat, REAfinity||Stem cells|
|GLAST (ACSA-1) Antibody, anti-human/mouse/rat||Astrocytes|
|CD11b Antibody, anti-human, REAfinity||Astrocytes|
|CD45 Antibody, anti-human, REAfinity||Microglia|
|O4 Antibody, anti-human/mouse/rat, REAfinity||Microglia|
|A2B5 Antibody, anti-human/mouse/rat||Oligodendrocytes|
|CD140a Antibody, anti-human, REAfinity||Oligodendrocytes|
|SSEA-1 Antibody, anti-human/mouse, REAfinity (CD15)||Oligodendrocytes|
|CD184 (CXCR4) Antibody, anti-human, REAfinity||Neurons|
|CD184 (CXCR4) Antibody, anti-human, REAfinity||Neurons|
|CD31 Antibody, anti-human, REAfinity||Endothelial cells|
PSC-derived brain organoids model rudimental brains that include multiple cellular populations, providing the opportunity to study connectivity and cellular interaction of neural cells. With our complete 3D imaging workflow, including 3D imaging techniques combined with tissue clearing, analyzing the structure and composition of whole brain organoids has never been easier.
Our antibodies for 3D-immunofluorescence (3D-IF) are already validated for 3D imaging of large samples such as organoids. Tailor-made to work with our MACS Clearing Kit and directly conjugated to bright and photostable Vio® Dyes, our recombinantly engineered REAfinity™ Antibodies offer you all the advantages of direct immunofluorescence microscopy: reduced staining time, great signal-to-noise ratio, and minimal background interference.
Suitable 3D-IF antibodies
|Sox2||Sox2 Antibody, anti-human/mouse, REAfinity Vio B515||Neural progenitor cells|
|Sox2 Antibody, anti-human/mouse, REAfinity Vio R667|
|OTX2||OTX2 Antibody, anti-human, REAfinity Vio B515||Embryonic Stem Cells / Neural progenitor cells|
|OTX2 Antibody, anti-human, REAfinity Vio R667|
|Ki-67||Ki-67 Antibody, anti-human/mouse, REAfinity Vio B515||General proliferation marker|
|Ki-67 Antibody, anti-human/mouse, REAfinity Vio R667|
The MACS Clearing Kit enables clearing of up to 60 PSC-derived cerebral organoids of approximately 1 mm3. By minimizing light scattering of opaque tissue, it allows to sharply image structures without compromising integrity. To make things even easier, we have generated a specific easy-to-follow tissue staining and clearing protocol that guides you through this hassle-free process step-by-step.
Light sheet microscopy enables the visualization of different cell populations without disrupting the macro-architecture of the brain organoid.
Discover our light sheet microscope family for 3D high-resolution imaging. Full automation of the UltraMicroscope Blaze™ Instrument enables overnight imaging of multiple samples in a single imaging experiment, without manual intervention or monitoring. The batch measurement mode ensures that 3D data will be available for in-depth analysis the next morning. Our pioneering UltraMicroscope technology combined with cutting-edge light sheet optics ensures excellent data quality from macro to subcellular imaging.
The video shows PSC-derived brain organoid labeled with Miltenyi Biotec recombinant antibodies (Ki-67 Antibody, Vio R667, REA183 (yellow); SOX2 Antibody, Vio B515, REA320 (magenta); and β-Tubulin 3 Antibody, Vio G570, REA1152 (cyan)), and optically cleared using the MACS Clearing Kit. Imaged with an UltraMicroscope Light Sheet Instrument and processed as a 3D-rendered maximum intensity projection.