Recombinant human IL-2 (interleukin 2) stimulates growth and differentiation of cells of the lymphoid lineage, such as T, NK, and B cells. IL-2 is a potent immunomodulatory cytokine, as it prevents autoimmunity and has key functions during infections. IL-2 IS stands for interleukin 2 "Improved Sequence", also termed aldesleukin, and is a variant of IL-2 with a serine substitution for the native cysteine at amino acid position 125. Human IL-2 IS is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays, and possesses the same biological properties as naturally occurring

Data and images for Human IL-2 IS

Figures

Figure 1

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Human IL-2 IS activity assay.
Activity of Human IL-2 IS, premium grade (red line) was compared to commercially available aldesleukin (black line).

Figure 1

Human IL-2 IS activity assay.
Activity of Human IL-2 IS, premium grade (red line) was compared to commercially available aldesleukin (black line).

Figure 2

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SDS-PAGE of Human IL-2 IS, premium grade
under non-reducing (NR) and reducing (R) conditions.

Figure 2

SDS-PAGE of Human IL-2 IS, premium grade
under non-reducing (NR) and reducing (R) conditions.

Figure 3

View details
Human IL-2 IS biological activity.
Activity of Human IL-2 IS, premium grade, (red bar) was compared to another commercially available product (black bar).

Figure 3

Human IL-2 IS biological activity.
Activity of Human IL-2 IS, premium grade, (red bar) was compared to another commercially available product (black bar).

Figure 4

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Mass spectrometry analysis (ESI-MS) of Human IL-2 IS, premium grade. The peak corresponds to the calculated molecular mass of 15400 Da.

Figure 4

Mass spectrometry analysis (ESI-MS) of Human IL-2 IS, premium grade. The peak corresponds to the calculated molecular mass of 15400 Da.

Specifications for Human IL-2 IS

Overview

Recombinant human IL-2 (interleukin 2) stimulates growth and differentiation of cells of the lymphoid lineage, such as T, NK, and B cells. IL-2 is a potent immunomodulatory cytokine, as it prevents autoimmunity and has key functions during infections. IL-2 IS stands for interleukin 2 "Improved Sequence", also termed aldesleukin, and is a variant of IL-2 with a serine substitution for the native cysteine at amino acid position 125. Human IL-2 IS is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays, and possesses the same biological properties as naturally occurring IL-2.

Applications

Human IL-2 IS can be used for a variety of applications including:
  • In vitro activation and propagation of T cells, e.g., in combination with the T Cell Activation/Expansion Kit, human.
  • In vitro stimulation of cytolytic function and expansion of NK cells, e.g., using the NK Cell Activation/Expansion Kit, human.
  • Generation of lymphokine-activated killer (LAK) cells or cytokine-induced killer (CIK) cells.

Detailed product information

Background information

IL-2, a potent lymphoid cell growth factor, is a typical four α-helix bundle cytokine. It is produced by activated T cells, especially the CD4
+
T helper cell population. It plays an important role in both the activation and maintenance of immune responses and in lymphocyte development. IL-2 promotes proliferation and differentiation of T cells, NK cells and B cells and is involved in the elimination of self-reactive T cells. IL-2 signals through a receptor complex consisting of IL-2 receptor α-chain (CD25), β- chain, and common γ-chain. The latter two are also used for IL-15 signaling.

Biological activity

  • Proliferation of CTLL-2 cells (NIBSC 86/504)
  • premium grade: ≥ 5×
    10
    6
    IU/mg
    (typical activity: ≥ 9×
    10
    6
    IU/mg
    )
  • research grade: ≥ 3×
    10
    6
    IU/mg

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade
cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific certificates of analysis are available on request (macstec@miltenyibiotec.de).

Resources for Human IL-2 IS

Documents and Protocols

Background information

Premium-grade cytokine benefits

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for Human IL-2 IS

Publications

  1. Pallett, L. J. et al. (2015) Metabolic regulation of hepatitis B immunopathology by myeloid-derived suppressor cells. Nat Med 21(6): 591-600
  2. Fuchs, S. et al. (2014)
    Patients with T
    +/low
    NK
    +
    IL-2 receptor γ chain deficiency have differentially-impaired cytokine signaling resulting in severe combined immunodeficiency.
    Eur. J. Immunol. 44(10): 3129-3140
  3. Engelmann S. et al. (2013) T cell-independent modulation of experimental autoimmune encephalomyelitis in ADAP-deficient mice. J. Immunol. 191(10): 4950-4959
  4. Valignat, M. P. et al. (2013) T lymphocytes orient against the direction of fluid flow during LFA-1-mediated migration. Biophys. J. 104(2): 322-331
  5. Fallarini S. et al. (2012) Invariant NKT cells increase drug-induced osteosarcoma cell death. Br J Pharmakon 167(7): 1533-1549
  6. Ng, S. B. et al. (2011) Dysregulated microRNAs affect pathways and targets of biologic relevance in nasal-type natural killer/T-cell lymphoma. Blood 118(18): 4919-4929
  7. Nguyen, T. L. et al. (2011) Antigen-specific TGF-β-induced regulatory T cells secrete chemokines, regulate T cell trafficking, and suppress ongoing autoimmunity. J. Immunol. 187(4): 1745-1753

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