Whether your focus relies on the fast isolation of pure and viable astrocytes from neonatal or adult rodent brain in half a day to astrocyte isolation and characterization with novel astrocyte-specific antibodies, our MACS Technology can support the efficiency of your workflows and fuel your achievements. By extending our solutions to the cultivation of primary astrocytes in serum-free media providing superior astrocyte growth even with low seeding densities is possible, to support your research needs, no matter the stage.
Video
Astrocyte research – novel tools for characterization, isolation, and cultivation
Melanie Jungblut gives an overview of novel approaches for the characterization and isolation of astrocytes and neural stem cells by combination of optimized cell preparation methods and sophisticated cell sorting technologies.
Pure cell suspensions from adult brain
The Adult Brain Dissociation Kit helps to remove cell debris and erythrocytes to acquire clean single cell suspensions for downstream isolation of astrocytes.
Highly viable neonatal astrocyte cell suspensions
The gentle dissociation of P4 mouse brain tissue with the Neural Tissue Dissociation Kit (P) results in a high percentage of viable cells.
Pure neonatal astrocytes in one hour
The MACS® MicroBead Technology in combination with Neural Tissue Dissociation Kits allows the isolation of pure neonatal astrocytes in one hour. Using astrocyte-specific MicroBeads based on the novel anti-GLAS (ACSA-1) or anti-ACSA-2 monoclonal antibodies results in purities over 90%.
Pure adult astrocytes in one hour
Highly pure and viable adult astrocytes can be isolated from single-cell suspensions obtained with the Adult Brain Dissociation Kit, mouse and rat by using the Anti-ACSA-2 MicroBead Kit, mouse.
Video
How to isolate pure viable astrocytes
Want to know the tips and tricks of how to get pure and viable astrocytes from adult brain in half a day? In our new video tutorial, our astrocytes expert from R&D shows you the entire workflow step by step, including:
- Automated dissociation of adult rodent brain tissue using the Adult Brain Dissociation Kit and gentleMACS™ Octo Dissociator with Heaters
- Isolation of pure, viable, and functional adult astrocytes from mouse brain in half a day, using MACS® Technology
- Downstream characterization and culture of isolated cells
Scientific poster
Efficient isolation of viable primary neural cells from adult murine brain tissue based on a novel automated tissue dissociation protocol
Hui Zhang1, Sandy Reiß1, Stefan Tomiuk1, Silvia Rüberg1, Richard Fekete2, Melanie Jungblut1, and Andreas Bosio1
1Miltenyi Biotec B.V. & Co. KG, Bergisch Gladbach, Germany; 2Fluidigm Corporation, South San Francisco, CA, USA
Superior astrocyte growth even with low seeding densities
Astrocytes cultured in AstroMACS Medium show an increased expansion rate. Hence, superior astrocyte growth is achieved even when seeded with low density.
Preservation of healthy astrocytes for downstream experiments
Neonatal astrocytes cultured in AstroMACS Medium show healthy astrocyte morphology
and cell-cell contacts.
Less dead cells and enhanced adult astrocyte culture
AstroMACS Medium allows enhanced adult astrocyte culture together with the new AstroMACS Separation Buffer, which removes dead cells during cell separation and provides a healthy culture environment.
Astrocyte-specific antibodies
Specific antibodies are essential for reliable neural cell studies, especially for cell types with high diversity, such as astrocytes. The monoclonal ACSA-2 and GLAST antibodies are pan antibodies and recognize both, non-reactive and reactive astrocytes.
- Jungblut, M. et al. (2012) Isolation and characterization of living primary astroglial cells using the new GLAST-specific monoclonal antibody ACSA-1. Glia 60 (6): 894–907.
- G. Kantzer, C. et al. (2017) Anti-ACSA-2 defines a novel monoclonal antibody for prospective isolation of living neonatal and adult astrocytes. Glia 65(6): 990–1004.
- Batiuk, M. Y. et al. (2017) An immunoaffinity-based method for isolating ultrapure adult astrocytes based on ATP1B2 targeting by the ACSA-2 antibody. J. Biol. Chem. 292(21): 8874–8891.
Isolation and cultivation of astrocytes from adult mouse brain
- Keywords
- Sample preparation
- Cell separation
- Cell culture
- Flow cytometry and cell analysis
- Neuroscience
- mouse
- brain
- astrocytes
- neural cells
- Application protocol
Isolation and cultivation of ACSA-2 positive astrocytes from neonatal mouse brain
- Keywords
- Sample preparation
- Cell separation
- Cell culture
- Flow cytometry and cell analysis
- Neuroscience
- mouse
- neural cells
- astrocytes
- Application protocol
Isolation and cultivation of GLAST-positive astrocytes from neonatal mouse brain
- Keywords
- Sample preparation
- Cell separation
- Flow cytometry and cell analysis
- Cell culture
- Neuroscience
- mouse
- brain
- neural cells
- astrocytes
- Application protocol
