A complete workflow solution for large-scale 3D fluorescence microscopy

See the big picture and dive deep into the details

High-resolution, 3D light sheet microscopy of large samples opens up new dimensions in the world of biomedical microscopy.  

Complex 3D structures, such as vasculatures or neuronal networks, can span from micrometers to centimeters. For this reason, they escape truly comprehensive analysis by current standard 2D and 3D microscopy techniques involving tissue sectioning. 

Miltenyi Biotec offers a complete workflow solution that allows you to analyze your intact sample in full and dive deep into the details. 

Acquiring meaningful 3D microscopy data from large samples such as organoids, whole rodent organs, or even human organs is a three-step process: 

1. The sample is stained with fluorochrome-conjugated antibodies. Thorough epitope labeling within the entire 3D structure and high signal-to-noise ratios are key for reliable analysis.
2. The tissue needs to be rendered transparent to allow excitation of fluorochromes deep inside the sample.
3. The signals are acquired with a microscope that can host large samples and has suitable optics with long working distance to generate both overview and high-resolution images of your large samples.

Miltenyi Biotec’s product portfolio dedicated to large-scale 3D imaging offers validated and ready-to-use protocols and reagents that provide you with the optimal performance when using our microscopes. Streamline your workflow – no matter if you are a microscopy expert or just about to start your 3D imaging project. 
 

Immuno-oncology

Miltenyi Biotec’s 3D imaging workflow offers many applications for immuno-oncology research, including 3D imaging of infiltrating lymphocytes in entire tumors. It also enables users to localize CAR T cells in a whole organism, and it can provide detailed insight into the tumor microenvironment through high-resolution imaging.

Neuroscience

For neuroscientists, UltraMicroscope light sheet imaging systems offer the option to generate 3D images of whole brains in unprecedented detail, facilitating studies on the pathology of Alzheimer’s and Parkinson’s diseases in mouse models, for example. 3D rendering allows the detailed analysis of complex structures within the brain, such as the vasculature.