Isolation, characterization, 
and cultivation of oligodendrocytes

  • Isolation of pure pre-mature oligodendrocytes (O4 positive) or oligodendrocyte precursor cells (OPCs) from neonatal brain tissue in an hour
  • Isolation of pure and viable pre-mature oligodendrocytes (O4 positive) from adult brain tissue in half a day
  • Neonatal or adult oligodendrocytes cultivation in serum-free medium

Application protocols

Discover our different workflows for oligodendrocytes and find the one that fits your experimental needs.
 

Isolation and cultivation of oligodendrocytes from adult mouse or rat brain

Isolation and cultivation of O4-positive oligodendrocytes from neonatal mouse or rat brain

Isolation and cultivation of oligodendrocyte precursor cells (OPCs) from neonatal mouse brain

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Brain (mouse)

Neural cells (mouse)

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Neuroscience (brochure)

Application data by workflow step

Dissociation of murine brain tissue

Debris and erythrocytes detected in cell suspensions before and after sample clearing

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Preparation of viable adult brain cells free of debris and red blood cells

Combined optimized enzymatic treatment using the Adult Brain Dissociation Kit with gentle mechanical dissociation using the gentleMACS™ Octo Dissociator with Heaters results in pure and viable single-cell suspensions. 
 

Purity and viability of cells obtained from murine (P4) whole brain tissue

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Standardized dissociation of neonatal neural tissues

Neural Tissue Dissociation Kits allow reliable tissue dissociation and result in a high yield of viable single cells.

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Magnetic isolation and flow cytometry analysis of oligodendrocytes

Single-cell suspension from P2 mouse brain before and after separation using MACS MicroBead Technology
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Isolation of oligodendrocyte precursor cells

Obtain pure, viable, and functional oligodendrocyte precursor cells by positive selection with the CD140a (PDGFRα) MicroBead Kit, mouse.

Single-cell suspensions from neonatal and adult brain before and after separation using MACS MicroBead Technology
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Isolation of pre-mature oligodendrocytes from neonatal or adult brain

High recovery of pre-mature oligodendrocytes from neonatal and adult brain with Anti-O4 MicroBeads, human, mouse, rat.

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Scientific poster
Efficient isolation of viable primary neural cells from adult murine brain tissue based on a novel automated tissue dissociation protocol

Hui Zhang1, Sandy Reiß1, Stefan Tomiuk1, Silvia Rüberg1, Richard Fekete2, Melanie Jungblut1, and Andreas Bosio1

1Miltenyi Biotec B.V. & Co. KG, Bergisch Gladbach, Germany; 2Fluidigm Corporation, South San Francisco, CA, USA

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Cultivation of oligodendrocytes

Cultivation of isolated oligodendrocytes
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Best culture conditions for oligodendrocytes

MACS® Neuro Medium supplemented with the serum-free MACS NeuroBrew®-21, PDGF-AA and FGF-2, are optimized to support growth and long-term survival of mouse oligodendrocytes. 
 

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Immunostaining and microscopy analysis of pre-mature oligodendrocytes

Anti-O4 staining of adult mouse oligodendrocytes
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Anti-O4 staining of isolated oligodendrocytes

Specific immunocytochemistry and immunohistochemistry staining of oligodenrocytes from adult mouse brain.

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