Discover our dynamic mesenchymal stem cell (MSC) workflows highlighting the importance of implementing effective and agile solutions from the beginning. Whether your interest demands optimized flow cytometry reagents for quantification and phenotyping analyses, our complete workflow support from MSC isolation and expansion with our xeno- and serum-free medium, to differentiation and immunomodulation, Miltenyi’s MSC solutions provide the efficiency and viability you need to succeed.
The MSC Enumeration Kit was developed for the standardized, quick, and absolute quantification of human MSCs from fresh bone marrow aspirate by flow cytometry. It takes only 30 minutes and requires only 200 µl of bone marrow sample per test.
The MSC Enumeration Kit, human provides a cocktail of 3 fluorochrome-conjugated antibodies that are used to identify MSCs, These include the MSC-specific marker CD271 (LNGFR) and exclusion markers CD45 and CD235a. Anti-MSCA-1 (W8B2) is provided separately in the kit and can be added to specifically identify MSCs with high proliferative potential.
Kathrin Godthardt, Claudia Schreiner, Andreas Bosio, and Sebastian Knöbel
Historically, a number of methods relying on specific physical properties have been used to isolate MSCs from sites at which they reside. The problem with this type of approach is that no physical properties have been uniquely ascribed to MSCs. Therefore, many different cell types are co-isolated, resulting in a mixed population of cells.
Magnetic cell isolation (positive selection) of CD271 (LNGFR)+ cells results in a cell population containing an approximately 1000-fold greater concentration of MSCs as compared to conventional methods of isolation. This enables isolation of a homogeneous, and consequently more effective non-hematopoietic stem cell population.
CD271 MicroBead Kits were developed for the isolation of CD271 (LNGFR)+ MSCs from bone marrow or adipose tissue with high yield and purity.
The Anti-MSCA-1 (W8B2) MicroBead Kit was developed for the isolation of MSCA-1 (W8B2)+ MSCs with high proliferative capacity from bone marrow aspirates.
Our cell culture options provide reliable solutions for the maintenance and expansion of MSCs with preserved differentiation potential and immunomodulation ability.
The MSC Phenotyping Kit was developed for the standardized identification and phenotyping of cultured human MSCs by flow cytometry based on the defined ISCT standards.
We provide a variety of media options that support the differentiation of MSCs into adipocytes, osteoblasts, and chondrocytes. The different StemMACS Differentiation Media are suitable for the analysis or quality control of the differentiation capacity of expanded MSCs, as well as in vitro studies focusing on the processes involved in MSC differentiation, including gene expression and protein profiling.
The suppression potential of MSCs varies among in vitro expanded cells and shows donor-dependent differences. MSCs can be “licensed” by inflammatory cytokines such IFN-γ and TNF-α to become more immunosuppressive and show a more homogeneous phenotype in this regard. The MSC Suppression Inspector, human was developed for standardized characterization of the immunomodulatory function of MSCs by co-culture with CD4+CD25– or CD4+ responder T (Tresp) cells.