Optics | |
| Laser excitation | 405 nm, 100mW 488nm, 100mW 638nm, 100mW |
| Emission detectors | V1: 450/50 nm V2: 525/50 nm B1: 525/50 nm B2: 585/40 nm B3: 655–730 nm B4: 750 nm LP R1: 655–730 nm R2: 750 nm LP Backscatter and side scatter for detection of size & granularity of cells |
| Fluorescence performance | 5 decade logarithmic scales, display in lin, log, or hlog scales |
Performance | |
| Purity | >99% |
| Cell flow rate | Up to 55,000 cells/s* for the MACS Tyto Cartridge (GMP & RUO) and 110,000 cells/s* for the MACS Tyto Cartridge HS (RUO) |
| Sort rate | Up to 30,000 valve actuations/s* |
| Viability | >99% for lymphocytes |
| Sorting mode | Two-way sorting of positive (sorted) cells and negative (non-sorted) cells |
| Technical support | Built-in live support functionality for reliable support in real time |
| Operating pressure | <230 mbar (3 psi) with the MACS Tyto Cartridge (GMP & RUO) and 1,100 mbar (17–18 psi) for the MACS Tyto Cartridge HS (RUO) |
| Temperature control range | 4–25 °C (under standard environmental conditions) |
| Carry-over | No sample-to-sample carry-over due to single-use disposable cartridge |
Data management | |
| Measurement parameters | Height measurements for all parameters |
| Signal processing | 16-bit analog-to-digital conversion and signal processing |
| Operating system | Core i7, 512 GB flash disk, 8 GB DDR4 RAM and Windows® 7 64-bit |
| Compensation | Full 8×8 matrix compensation |
| Data files | .mqd (proprietary file type); fcs (2.0, 3.0, 3.1 compatible) |
Operation details | |
| Dimensions | 965 × 585 × 280 mm3: without external monitor 965 × 585 × 760 mm3: with external monitor |
| Weight | 62 kg (137 lbs): without external monitor 67 kg (148 lbs): with external monitor |
| Monitors | 32 ̎ display and 15.6 ̎ touchscreen (internal) |
| Power requirements / consumption | 100-240 V, 50/60 Hz / 300W: without external monitor 100-240 V, 50/60 Hz / 500W: with external monitor |
| Ports | 4× USB 2.0, 6× USB 3.0, 2× Ethernet, DVI-D |
Environmental conditions | |
| Operational temperature / humidity | 18–25 °C / 0–85% relative humidity, non-condensating |
| Emission sound pressure < 70 dB(A) level at workstation | <70 dB(A) |
Cartridge details | |
| Sterility | ETO validated sterilization method to guarantee sterility of each cartridge |
| A-septic filters | 0.1 µm hydrophobic filters at air inlet and outlet ports |
| Sorting channel width | 25 × 50 µm |
| Maximum loading volume (input) | 100 µL–10 mL |
| Maximum loading cell number | 5×107 cells/mL |
| Cartridge alignment for laser positioning | Continuous automated cartridge alignment also during the sort |
| Cell retrieval | Negative sorted cells are kept in the negative collection chamber |
| Barcode labeling | Cartridges are labeled with a barcode for easy identification and traceability |
*dependent on input sample and Poisson statistics
- MACSQuant® Tyto® Cell Sorter – User manual
- MACSQuantify™ Tyto® Software 2.0 – User manual
- MACSQuantify™ Tyto® Software 1.0 – User manual
- MACSQuantify™ Tyto® Software 0.5 – User manual
- MACSQuantify™ Tyto® Software 0.4 – User manual
- MACSQuantify™ Tyto® Software 0.3.1 – User manual
- MACSQuantify™ Tyto® Software 0.3.1 – Short instructions
Brochures/posters
- MACS® GMP Tyto® Consumables - GMP-compliant cell sorting with the MACSQuant® Tyto System
- MACSQuant® Tyto® Cell Sorter - The revolution in cell sorting has begun
Scientific posters
- Isolation of antigen specific CD8+ T cells using the MACSQuant® Tyto® : A closed, sterile, single use Microchip based cell sorter
- Towards optimal neutrophil purification.
- Microchip-based fluorescence-activated cell sorting of antigen-specific CD137+CD8+ T cells in a disposable and closed cartridge system using the MACSQuant® Tyto® Sorter
- REAlease® Fluorochrome Technology for multiparameter cell sorting
- A novel approach for gentle sorting of adult neural stem cells from wild type mouse brain using MACSQuant® Tyto®
- GMP-compliant flow cytometric cell sorting of antigen-specific T cells using MACS® GMP CD8-APC and MACS GMP CD137-PE Fluorescent Antibodies on the MACSQuant® Tyto® Cell Sorter
- GMP-compliant flow cytometric cell sorting of antigen-specific T cells using MACS® GMP CD8-APC and MACS GMP CD137-PE Fluorescent Antibodies on the MACSQuant® Tyto® Cell Sorter
- How to make a fishing rod for adoptive cellular therapy: the ACTolog® approach, a multi-targeted endogenous T-cell therapy
- GMP-compliant microchip based cell sorting of IPSCS-derived dopaminergic progenitors
- Highly efficient dissociation of distinct adult rodent brain regions ensures high cell viability and recovery for successful downstream processing
- Standardized generation, purification, and quality control of human pluripotent stem cell–derived cardiomyocytes
- Sorting of MSCs with CD271 and MSCA-1 antibodies in a closed-cartridge system using the MACSQuant® Tyto® Sorter
- Microchip-based sorting of high-risk material s in a disposable and fully closed cartridge system using the MACSQuant® Tyto® Sorter
- REAlease® Technology: controlled release of antibody-fluorochrome conjugates for maximal flexibility in flow sorting applications
App notes/customer reports
- MACSQuant® Tyto® Cell Sorter Sorting of rare SARS-CoV-2-specific B cells.
- Advance your T cell research with a closed and sterile system - Isolation of human regulatory T cells (Tregs) with the CliniMACS Prodigy® and the MACSQuant® Tyto® Cell Sorter
- MACSQuant® Tyto® Cell Sorter and DispenCell - Combining cell sorting and single-cell deposition
- Isolation of CD8+ antigen-specific T cells using the MACSQuant® Tyto® Cell Sorter
- Sorting and cultivation of adult neural stem cells from mice
- Gentle sorting of PBMC populations
- Complete workflow to sort highly pure and viable sub-populations of TILs from a single mouse tumor sample
Tyto instrument introduction
Play video
Tyto applications
- Carstensen, L. et al. (2019) Long-Term Exposure to Inflammation Induces Differential Cytokine Patterns and Apoptosis in Dendritic Cells. Front Immunol 10(2702): 2709
- Burgoyne, P. et al. (2021) J. Leukoc. Biol. 111(6): 1243-1251CCR7+dendritic cells sorted by binding of CCL19 show enhanced Ag-presenting capacity and antitumor potency.
- Radke, S. et al. (2020) Mol Ther Methods Clin Dev. 18: 679-691Purification of Human CD34+CD90+HSCs Reduces Target Cell Population and Improves Lentiviral Transduction for Gene Therapy.
- Ötztürk, . E. et al. (2021) scAAVengr, a transcriptome-based pipeline for quantitative ranking of engineered AAVs with single-cell resolution. Elife 10: e64175
- Bacher, P. et al. (2020) Immunity 53: 1258-1271Low avidity CD4+T cell responses to SARS-CoV-2 in unexposed individuals and humans with severe COVID-19.
- Jonsson, A. et al. (2020) Transcriptional profiles of human islet and exocrine endothelial cells in subjects with or without impaired glucose metabolism. Sci Rep 10(1): 22315
- Zhou, H. et al. (2021) Identifying the functions of two biomarkers in human oligodendrocyte progenitor cell development. J. Transl. Med. 19(1): 188
- Dugan, H. L. et al. (2021) Profiling B cell immunodominance after SARS-CoV-2 infection reveals antibody evolution to non-neutralizing viral targets. Immunity 54(6): 1290-1303
- Roberts, L. M. et al. (2021) Cutting Edge: Lung-Resident T Cells Elicited by SARS-CoV-2 Do Not Mediate Protection against Secondary Infection. J Immunol 207(10): 2399-2404
- Shu, R. et al. (2021) Engineered CAR-T cells targeting TAG-72 and CD47 in ovarian cancer. Mol Ther Oncolytics 20: 325-341
- Fattorelli, N. et al. (2021) Stem-cell-derived human microglia transplanted into mouse brain to study human disease. Nat Protoc. 16(2): 1013-1033
- Ou, K. et al. (2021) Strong Expansion of Human Regulatory T Cells for Adoptive Cell Therapy Results in Epigenetic Changes Which May Impact Their Survival and Function. Front Cell Dev Biol. 9: 751590
- Marongiu, L. et al. (2022) Maturation signatures of conventional dendritic cell subtypes in COVID-19 suggest direct viral sensing. Eur J Immunol. 52(1): 109-122
- Zazhytska, M. et al. (2022) Non-cell-autonomous disruption of nuclear architecture as a potential cause of COVID-19-induced anosmia. Cell 185(6): 1052-1064
- Haruna, M. et al. (2022) Sci Rep 12(1): 5377The impact of CCR8+regulatory T cells on cytotoxic T cell function in human lung cancer.
- Wang, Z. et al. (2022) Efficient recovery of potent tumour-infiltrating lymphocytes through quantitative immunomagnetic cell sorting. Nat Biomed Eng 6(2): 108-117
- Ocanas, S. R. et al. (2022) eNeuro 9(2): 0348Minimizing theEx VivoConfounds of Cell-Isolation Techniques on Transcriptomic and Translatomic Profiles of Purified Microglia.
Cell sorting has never been easier with the MACSQuantify™ Tyto Software
- Press play and walk away: No hands-on during sort process
- No additional skills needed: Sorting is as simple as operating a MACSQuant® Analyzer
- Maximal flexibility in panel design and usage of bright and dim markers
- High sort rates with increased yield and purity
- Automatically generated run reports including real-time sort statistics and instrument settings
Support at your fingertips
- MACSQuant Live Support for real-time assistance from our experts
- Training at Miltenyi University
- Comprehensive service options provided by our globally distributed field service teams
MACSQuant® Tyto® Cell Sorter
The revolution in cell sorting has begun
Order no.: 130-103-931. For research use only.
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