Optics | |
| Laser excitation | Spatially separated: 405 nm, 40 mW 488 nm, 30 mW 640 nm, 20 mW |
| Emission detectors | FSC: 488/10 nm SSC: 488/10 nm V1: 450/50 nm V2: 525/50 nm B1: 525/50 nm B2: 585/40 nm B3: 655–730 nm B4: 750 nm LP R1: 655–730 nm R2: 750 nm LP |
| Fluorescence sensitivity and resolution | MESFs (CV <5%): FITC <200 PE <100 APC <150 |
| Flow cell dimensions | 200×250 μm |
| Fluorescence detectors | Optimized with spectrally matched PMTs for all channels |
Fluidics | |
| Minimal uptake volume1 | 1 μL (25 μL recommended for volumetric counting applications) |
| Sample flow rate | 25, 50, or 100 µL/min or automate flow rate to maintain 500, 1000, or 2000 events/second |
| Measurement speed2,3 | <25 minutes per 96-well plate (5 μL measurement volume per well) |
| Sample uptake | 1–450 µL |
| Maximal event rate | Up to 15,000 events/second |
Technical specifications | |
| Footprint | 385.5 × 284.5 mm (15.18 × 11.2 in) |
| Size (width×depth) Height (adjustable touchscreen) | 669 × 400 mm (26.34 × 15.75 in) 394–553.5 mm (15.51–21.79 in) |
| Size with miniSampler Plus (width × depth) | 669×500 mm (26.34 × 19.69 in) |
| Weight | 50 kg (110 lbs) |
| Monitor | 15.6" LCD touchscreen |
| Power requirements | 100–240 V~, 50/60 Hz |
| Power consumption (standard use) | 450 W |
| RAM | 8 GB DDR4 (SO-DIMM) |
| Mass storage | 500 GB SSD |
| Ports | 4× USB 2.0 ports, 6× USB 3.0 ports (2 at display), 2× DisplayPort, 2× LAN, DVI, RE-232, Audio |
| Emission sound pressure level at workstation | <61 dB(A) |
Performance | |
| Sample carry-over2,4 | 0.01% |
| Fluorescence performance | 5-decade logarithmic scale (10-2 to 103), displayed in lin, log, or hlog scales |
Data management | |
| Operating system | Embedded operating system |
| Measurement parameters | Area, width, height for all parameters, with time and volume |
| Signal processing | >18 bit dynamic range in area with 32 bit floating point signal processing |
| Compensation | Automated, or manual with 8×8 matrix, during or after acquisition |
| Data files | .mqd (proprietary file format), .fcs (2.0, 3.0, 3.1 compatible) |
| 1 At every uptake, an additional excess volume is aspirated by the instrument. The excess volumes are calibration- and process-dependent and do not exceed 10 μL for Fast, Standard, and Extended modes, and 20 μL for Screen mode. 2 Referred value indicates the average of multiple experiments and can differ for individual sample materials. 3 The measurement speed is determined by measuring the time between the movement of the robotic arm into the first measured well, and its movement out of the last measured well. The measurements were carried out at high flow rate in fast mode. 4 For carry-over, full 96-well plates were loaded with 200 μL/well of PBMC suspension at a nominal concentration of 10,000 cells/μL in every other well ("SRC-wells”). Alternating wells are loaded with an equal volume of MACSQuant® Running Buffer ("CO-wells”). The uptake volume was set to 100 μL and measured at medium flow rate in standard mode. The carry-over is defined by sum (CO-singlet count)/sum(SRC-singlet count) ×100%. | |
- MACSQuant Instrument user manual
- MACSQuantify Software 2.8 user manual
- MACSQuantify Software 2.10 user manual
- MACSQuantify Software 2.11 user manual
- MACSQuantify Software 2.13 user manual
- MACSQuantify™ Maintenance - short instructions
- MACSQuantify™ Start-up - short instructions
- MACSQuantify™ PMT calibration - short instructions
- MACSQuantify™ Multisampling - short instructions
- MACSQuantify™ Icons - short instructions
- MACSQuantify™ File management- short instructions
- MACSQuantify™ Acquisition - short instructions
- MACSQuantify™ Photomultiplier tube (PMT) calibration - short instructions
Brochures/posters
Scientific posters
- A novel user-independent CFU assay for hematopoietic stem and progenitor cells
- Spatial information on receptor clustering in flow cytometry using FRET
- GMP-compliant flow cytometric cell sorting of antigen-specific T cells using MACS® GMP CD8-APC and MACS GMP CD137-PE Fluorescent Antibodies on the MACSQuant® Tyto® Cell Sorter
- Sensitive monitoring of CAR+ T cells in peripheral blood by flow cytometry and real-time qPCR
- Standardization of whole blood immunophenotyping using an automated staining protocol and recombinant antibodies
- Method validation of a flow cytometry assay for sensitive detection of CD20 CAR+ T cells in peripheral blood
- Standardized and flexible eight-color flow cytometry panels harmonizedbetween different laboratories to study human NK cell phenotype and function
App notes/customer reports
- PSC-derived cardiomyocyte subtypes
- MACSxpress® Technology allows isolation of B cells with high purity for sensitive and concordant microarray-based gene expression profiling.
- Comprehensive flow cytometry Analysis of isolated NK cells from whole blood.
- Characterization using the MACSQuant Analyzer 10 - Analysis of NKT cell endogenous ligands.
- Reliable, hands-free, and high-throughput cell viability assays on the MACSQuant® Flow Cytometer
- No-lyse whole blood Analysis- Simplify flow cytometric analyses with the MACSQuant® Analyzer
- Detection, enrichment, and direct phenotypic and functional characterization of antigen-specific naive and memory T cell subsets.
- Comparison between antibodies from different providers: determination of median fluorescent intensities and stain indices.
- High-throughput genotoxicity assays
- A simple no-wash method for the flow cytometric enumeration of leukocyte subsets in whole blood.
- Identification of different Plasmodium falciparum life cycle stages in human red blood cells using the MACSQuant Analyzer.
- Determination of ploidy levels in plant cells - Applications for the MACSQuant® Analyzer
- Characterization of microvesicles using the MACSQuant® Analyzer.
- Generation of functional CAR T cells from CD4+ and CD8+ T cells
- Modulation of allergic airway inflammation by the histamine H4 receptor: analysis by flow cytometry.
- High-sensitivity detection and analysis of rare cells by integrated magnetic enrichment and flow cytometry using the MACSQuant® Analyzer.
- Automated compensation of the MACSQuant® Instrument
- Magnetic enrichment of antigen-specific CD4+ T cells using the MACSQuant® Analyzer 10 enables the in-depth characterization of vaccine-induced circulating follicular T helper cells.
- Automated isolation of CD56+ natural killer cells and antibody-dependent cellular cytotoxicity.
- IPC/QC of gene-engineered HSCs manufactured by the CliniMACS Prodigy®
- Measurement assurance and harmonization using the MACSQuant® Analyzer in cell counting applications
- Antibody panel building guide
- How to use a MACSQuant® Instrument Express Mode in Custom Login
- MACSPlex Analysis of plasma-derived extracellular vesicles
- Determination of intracellular antibody production, cell density, and viability of recombinant CHO-DG44 cells using the MACSQuant Analyzer.
- Sorting and cultivation of adult neural stem cells from mice
- Characterization of exosome populations using the MACSQuant® Analyzer
- Efficient and rapid in vitro generation of fully functional multi-virus-specific CD4+ and CD8+ T cells.
- FRET Express Mode
- Magnetic enrichment of antigen-specific CD4+ T cells enables the in-depth characterization of vaccine-induced circulating follicular T helper cells.
- Identification and enumeration of melanoma-specific tumor-infiltrating lymphocytes and cancer stem cells.
- MACSQuant® Flow Cytometers: MACSPlex Filter Plate calibration
- In Vitro MicroFlow® Kits and MACSQuant® Analyzer 10: High-throughput genotoxicity assays
- Applications for the enrichment column of the MACSQuant® Analyzer: Enumeration of circulating endothelial cells
Protocols
Other downloads
The seamless integration of the MACSQuant 10 Flow Cytometer into our liquid handling system allowed us to improve the reproducibility of our cytometry experiments and dramatically improved our productivity.
Chris Groves, Ph.D.
Senior Manager, R&D BioPharmaceuticals
AstraZeneca, Gaithersburg, USA
AstraZeneca, Gaithersburg, USA
Harmonizing the world is just an instrument setting away with MACSQuantify™ Software
- Easy assay transfer from one instrument to another for comparable, standardized results and multi-instrument alignment during inter-lab collaborations
- Express modes with predefined experiment settings as well as acquisition and analysis templates
- Automated fluorescence compensation
- Compliance with 21 CFR Part 11 for data submission to regulatory agencies
- Audit trail, analysis reports with e-signature, and user management system according to 21 CFR Part 11
Support at your fingertips
- MACSQuant Live Support for real-time assistance from our experts
- Technical and field application support for assay design, custom automation, and express mode development
- Training at Miltenyi University
- Comprehensive service options provided by our globally distributed field service teams
MACSQuant® Analyzer 10 Flow Cytometer
Your trusted partner in automated flow cytometry
Order no.: 130-096-343. For research use only.
Related products
for
MACSQuant® Analyzer 10
MACSQuant
® Analyzer 10
