Untouched isolation of PBMCs without density gradient centrifugation

  • Get untouched PBMCs directly from whole blood in 25 min 
  • Easy and fast protocol without density gradient centrifugation 
  • Pure and viable PBMCs for various downstream applications 

The MACSprep™ PBMC Isolation Kit has been developed for the fast isolation of human peripheral blood mononuclear cells (PBMCs) from 1-8 mL of freshly drawn anticoagulated whole blood without density gradient centrifugation. Isolated PBMCs are suitable for various downstream applications, such as co-cultures like functional immune cell assays, cytokine expression studies, or immune monitoring.

Basic principle

Basic principle of PBMC isolation using the MACSprep™ PBMC Isolation Kit
View details

With MACSprep™ PBMC Isolation Kits, untouched isolation of human PBMCs is performed in three easy steps, including labeling of non-PBMCs and a two-step separation procedure.

Data

Untouched isolation of PBMCs from human whole blood

Untouched PBMCs from high viability and purity can be isolated from human whole blood using the MACSprep™ PBMC Isolation Kit, a MACSmix™ Tube Rotator, and a LS Column

Before separation

Lysed whole blood gated on CD45+ cells

After separation

Gated on CD45+ cells
Gated on viable CD45+ cells
Gated on viable CD45+ cells
Gated on viable CD45+ CD3+ cells

Untouched PBMCs were isolated from 10 mL of human EDTA-anticoagulated whole blood using the MACSprep™ PBMC Isolation Kit, a MACSmix™ Tube Rotator, and an LS Column. The isolated cells were fluorescently stained with CD45-VioBlue® , CD3-FITC, CD4-VioGreen™, CD8-APC-Vio®  770, CD14-APC, CD16-PE, CD56-PE, CD20-PE-Vio770, and 7-AAD and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris, non-leukocytes, and dead cells were excluded from the analysis based on CD45 expression, scatter signals, and 7-AAD.

Related information

Matching products: