Vio® Bright R720 and Vio R720 fluorophores 

Brighter and clearer staining for the red laser

The multimerization technology for Vio Bright dyes results in a 2-fold or higher increase in brightness of Vio Bright R720 compared to a conventional fluorophore like Alexa Fluor 700. Increased brightness of Vio Bright R720 conjugates is observed across different antigens; the data below show examples of CD19 and CD56 staining. Greater brightness of Vio Bright R720 compared to Alexa Fluor 700 is also reflected in the values of mean fluorescence intensity (MFI) and stain index (SI) for the detection of specific cells.

The stability of both Vio R720 and Vio Bright R720 upon exposure to ambient light was analyzed at different time points. Analysis of the photostability of a CD4 antibody conjugated to Vio R720 and Vio Bright R720 showed no major change in both MFI and SI after up to 24 hours of continuous exposure to ambient light (~850 lux), which is similar to other commercially available alternatives.  

Multicolor flow cytometry panels

Miltenyi Biotec–tested panels (MBTPs) are a collection of multicolor antibody panels that are optimized and pre-designed for specific flow cytometry applications. They are developed and validated by Miltenyi Biotec’s R&D experts, as well as by the customers who actually use Miltenyi Biotec antibodies. 

• MBTP 32: Immunophenotyping of human γδ T cells using flow cytometry
• MBTP 33: Immunophenotyping of activated T cells within antigen-stimulated human PBMCs using flow cytometry