Clone:
REA143
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IF, IHC, MC
Alternative names:
HLA-B, AS, SPDA1

Extended validation for HLA Class I Bw6 Antibody, anti-human, REAfinity™

Specificity

Knockout validation
To ensure antibody specificity, the target gene is knocked out in a suitable cell line using the CRISPR/Cas9 system and the knockout is confirmed by sequencing of the target locus. The antibody is considered to bind specifically to the intended epitope if no antibody binding to the knockout cells can be detected. The antibody staining is controlled by fluorescence microscopy and/or flow cytometry.
WT
KO
View details
Fluorescence microscopy image of HLA Class I Bw6 knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with HLA Class I Bw6-PE (REA143, red) and counterstained with DRAQ5 (blue) as DNA stain.
View details
Fluorescence microscopy image of HLA Class I Bw6 knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with HLA Class I Bw6-PE (REA143, red) and counterstained with DRAQ5 (blue) as DNA stain.
Fluorescence microscopy image of HLA Class I Bw6 knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with HLA Class I Bw6-PE (REA143, red) and counterstained with DRAQ5 (blue) as DNA stain.
View details
Overlay histogram showing flow cytometric analysis of HLA Class I Bw6 knockout cells. Wild type (red) and knockout cells (blue) were stained with CD143-PE, clone (REA143). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Overlay histogram showing flow cytometric analysis of HLA Class I Bw6 knockout cells. Wild type (red) and knockout cells (blue) were stained with CD143-PE, clone (REA143). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for HLA Class I Bw6 Antibody, anti-human, REAfinity™

Overview

Clone REA143 recognizes HLA Bw6, a class I human leukocyte antigen (HLA). Expressed on the surface of most nucleated cells, class I molecules are heterodimeric molecules and consist of a type I integral membrane α heavy chain and soluble β2 microglobulin protein. The extracellular region of the heavy chain further consists of 3 domains, of which one comprises the peptide binding groove. Antigens binding to class I molecules are 8–10 amino acids long and play an important role in recognition of the virus infected and malignant cells by cytotoxic T lymphocytes (CTLs). In addition, class I molecules interact with NK cell receptors to modulate the activity of NK cells. Downregulation of HLA class I Bw6 allele has been reported in leukemic patients where it is suggested that via this downregulation, leukemic cells may circumvent attack by CTLs.
Additional information: Clone REA143 displays negligible binding to Fc receptors.

Alternative names

HLA-B, AS, SPDA1

Detailed product information

Technical specifications

CloneREA143
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hosthuman cell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenHLA Class I Bw6
Alternative names of antigenHLA-B, AS, SPDA1
Molecular mass of antigen [kDa]32
Entrez Gene ID3106
RRIDAB_2819460, AB_2819663, AB_2652025, AB_2652026, AB_2652027, AB_2652028, AB_2652029, AB_2652030, AB_2652031, AB_2652032, AB_2652035, AB_2652036, AB_2652037, AB_2652038, AB_2905244, AB_2905243, AB_2905246, AB_2905245, AB_2922088, AB_2922055, AB_2905242, AB_2905241, AB_2819475

Resources for HLA Class I Bw6 Antibody, anti-human, REAfinity™

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for HLA Class I Bw6 Antibody, anti-human, REAfinity™

Publications

  1. Demanet, C. et al. (2004) Down-regulation of HLA-A and HLA-Bw6, but not HLA-Bw4, allospecificities in leukemic cells: an escape mechanism from CTL and NK attack? Blood 103: 3122-3130
  2. Hewitt, E. W. et al. (2003) The MHC class I antigen presentation pathway: strategies for viral immune evasion. Immunology 110(2): 163-169
  3. Jung, C. J. et al. (2015) Comparative analysis of piggyBac, CRISPR/Cas9 and TALEN mediated BAC transgenesis in the zygote for the generation of humanized SIRPA rats. PLoS Pathog. 11: 31455

Related products for
HLA Class I Bw6 Antibody, anti-human, REAfinity™

3 products available