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Isolation of CD141 (BDCA-3) ++ myeloid dendritic cells from PBMCs using the CD141 (BDCA-3) MicroBead Kit, a MiniMACS™ Separator, and MS Columns. Cells were stained with CD141 (BDCA-3)-PE and CD14-FITC. Only very few CD1c (BDCA-1) + myeloid dendritic cells, CD303 (BDCA-2) + plasmacytoid dendritic cells, and CD14 + monocytes are coenriched, although they express CD141 (BDCA-3) at low levels. |
PBMCs before separation | Enriched CD141 (BDCA-3) ++ myeloid dendritic cells |
CD141 (BDCA-3) MicroBead Kit, humanFigure 1Isolation of CD141 (BDCA-3) ++ myeloid dendritic cells from PBMCs using the CD141 (BDCA-3) MicroBead Kit, a MiniMACS™ Separator, and MS Columns. Cells were stained with CD141 (BDCA-3)-PE and CD14-FITC. Only very few CD1c (BDCA-1) + myeloid dendritic cells, CD303 (BDCA-2) + plasmacytoid dendritic cells, and CD14 + monocytes are coenriched, although they express CD141 (BDCA-3) at low levels. | CD141 (BDCA-3) MicroBead Kit, humanFigure 1Isolation of CD141 (BDCA-3) ++ myeloid dendritic cells from PBMCs using the CD141 (BDCA-3) MicroBead Kit, a MiniMACS™ Separator, and MS Columns. Cells were stained with CD141 (BDCA-3)-PE and CD14-FITC. Only very few CD1c (BDCA-1) + myeloid dendritic cells, CD303 (BDCA-2) + plasmacytoid dendritic cells, and CD14 + monocytes are coenriched, although they express CD141 (BDCA-3) at low levels. |
Isolation of CD141 (BDCA-3) ++ myeloid dendritic cells from PBMCs using the CD141 (BDCA-3) MicroBead Kit, a MiniMACS™ Separator, and MS Columns. Cells were stained with CD141 (BDCA-3)-PE and CD14-FITC. Only very few CD1c (BDCA-1) + myeloid dendritic cells, CD303 (BDCA-2) + plasmacytoid dendritic cells, and CD14 + monocytes are coenriched, although they express CD141 (BDCA-3) at low levels. |
PBMCs before separation | Enriched CD141 (BDCA-3) ++ myeloid dendritic cells |
CD141 (BDCA-3) MicroBead Kit, humanFigure 1Isolation of CD141 (BDCA-3) ++ myeloid dendritic cells from PBMCs using the CD141 (BDCA-3) MicroBead Kit, a MiniMACS™ Separator, and MS Columns. Cells were stained with CD141 (BDCA-3)-PE and CD14-FITC. Only very few CD1c (BDCA-1) + myeloid dendritic cells, CD303 (BDCA-2) + plasmacytoid dendritic cells, and CD14 + monocytes are coenriched, although they express CD141 (BDCA-3) at low levels. | CD141 (BDCA-3) MicroBead Kit, humanFigure 1Isolation of CD141 (BDCA-3) ++ myeloid dendritic cells from PBMCs using the CD141 (BDCA-3) MicroBead Kit, a MiniMACS™ Separator, and MS Columns. Cells were stained with CD141 (BDCA-3)-PE and CD14-FITC. Only very few CD1c (BDCA-1) + myeloid dendritic cells, CD303 (BDCA-2) + plasmacytoid dendritic cells, and CD14 + monocytes are coenriched, although they express CD141 (BDCA-3) at low levels. |
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