Miltenyi Biotec-tested panel 35 (MBTP 35)
This application protocol describes the characterization of human monocytes, neutrophils, eosinophils, and T, B, and NK lymphocyte populations as well as CD4+, CD8+, and CD56+CD3+ T cell subsets in human ovarian carcinoma using flow cytometry. Prior to staining the tumor was dissociated employing the gentleMACS™ Octo Dissociator with Heaters and Tumor Dissociation Kit, human.
Specificity | Clone | Purpose | Fluorochrome | Detection filter (nm) (laser) |
CD3 | REA613 | T cells | PE | 579/34 (blue) |
CD4 | REA623 | T helper cells | Vio® Bright 667 | 667/30 (red) |
CD8 | REA734 | Cytotoxic T cells | APC-Vio 770 | 785/62 (red) |
CD14 | REA599 | Monocytes | VioBlue® | 450/50 (violet) |
CD16 | REA423 | Neutrophils, eosinophils | Vio Bright 515 | 525/50 (blue) |
CD19 | REA675 | B cells | PE-Vio 770 | 785/62 (blue) |
CD45 | REA747 | Leukocytes | VioGreen™ | 525/50 (violet) |
CD56 | REA196 | NK cells | Vio Bright 515 | 525/50 (blue) |
7-AAD Staining Solution | - | Viability | - | 667/30 (blue) |
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