Immunophenotyping of human CD4+ and CD8+ T cell activation using flow cytometry

MBTP 54  = Miltenyi Biotec-tested panel 54

This application protocol describes the analysis of human CD4+ and CD8+ T cell activation markers after T cell stimulation. To demonstrate this state-of-the-art panel for CD4 T cell activation as well as CD8 T cell activation, PBMCs were stimulated for 48 hours using Miltenyi Biotec’s T Cell TransAct™, human. The activation state was defined by the expression level of HLA-DR, CD25, CD69, and CD38.

Protocol

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Materials

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