Miltenyi Biotec-tested panel 44 (MBTP 44)
This application protocol describes the characterization of CAR T cell functionality in terms of effector cytokine production upon antigen encounter using flow cytometry. To demonstrate the state-of-the-art panel, CAR T cells were co-cultured with antigen-expressing or antigen-negative target cells (GFP+) in presence of Brefeldin A.
|Specificity||Clone||Purpose||Fluorochrome||Detection filter (nm) (laser)|
|Viobility™ 405/452 Fixable Dye||-||Viability||VioGreen™||525/50 (violet)|
|CD3||REA613||T cells||VioBlue®||450/50 (violet)|
|CD8||REA734||Cytotoxic T cells||PE-Vio® 615||655-730 (blue)|
|IFN-γ||45-15||Effector functions||APC-Vio 770||750 LP (red)|
|TNF-α||REA656||Effector functions||APC||655-730 (red)|
|IL-2||REA689||Effector functions||PE-Vio 770||750 LP (blue)|
|CD19 CAR Detection Reagent, Biotin||-||CAR+ cells||-||-|
|Biotin||REA746||CAR+ cells||PE||585/40 (blue)|
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