Pluripotent Stem Cell MicroBeads were developed for the separation of human pluripotent stem cells based on the expression of the CD326 (EpCAM) antigen.
The Pluripotent Stem Cell MicroBeads can be used for the:
  • positive selection of undifferentiated CD326+ pluripotent stem cells, for example, human embryonic stem (ES) cells and induced pluripotent stem (iPS) cells
  • enrichment of iPS cells after reprogramming

Data and images for Pluripotent Stem Cell MicroBeads, human

Figures

Figure 1

Undifferentiated CD326
+
iPS cells were isolated from cultures containing spontaneously differentiated iPS cells using Pluripotent Stem Cell MicroBeads, an LS Column, and a MidiMACS™ Separator. Cells were fluorescently stained with CD326 (EpCAM)-APC, Stemgent® Phycoerythrin (PE) Anti-Human TRA-1-60 Antibody, and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Unseparated fraction
Enriched CD326
+
pluripotent cells
View details

Figure 1

Undifferentiated CD326
+
iPS cells were isolated from cultures containing spontaneously differentiated iPS cells using Pluripotent Stem Cell MicroBeads, an LS Column, and a MidiMACS™ Separator. Cells were fluorescently stained with CD326 (EpCAM)-APC, Stemgent® Phycoerythrin (PE) Anti-Human TRA-1-60 Antibody, and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
View details

Figure 1

Undifferentiated CD326
+
iPS cells were isolated from cultures containing spontaneously differentiated iPS cells using Pluripotent Stem Cell MicroBeads, an LS Column, and a MidiMACS™ Separator. Cells were fluorescently stained with CD326 (EpCAM)-APC, Stemgent® Phycoerythrin (PE) Anti-Human TRA-1-60 Antibody, and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for Pluripotent Stem Cell MicroBeads, human

Overview

Pluripotent Stem Cell MicroBeads were developed for the separation of human pluripotent stem cells based on the expression of the CD326 (EpCAM) antigen.
The Pluripotent Stem Cell MicroBeads can be used for the:
  • positive selection of undifferentiated CD326+ pluripotent stem cells, for example, human embryonic stem (ES) cells and induced pluripotent stem (iPS) cells
  • enrichment of iPS cells after reprogramming

Detailed product information

Background information

CD326 is expressed on undifferentiated human embryonic stem (ES) cells and induced pluripotent stem (iPS) cells and is localized to OCT4
+
pluripotent cells. Its expression is down-regulated upon differentiation.
1,2

Columns

LS or autoMACS
®
Columns.

References for Pluripotent Stem Cell MicroBeads, human

Publications

  1. Ng, V. Y. et al. (2010) Characterization of epithelial cell adhesion molecule as a surface marker on undifferentiated human embryonic stem cells. Stem Cells 28: 29-35
  2. Sundberg, M. et al. (2009) CD marker expression profiles of human embryonic stem cells and their neural derivatives, determined using flow-cytometric analysis, reveal a novel CD marker for exclusion of pluripotent stem cells. Stem Cell Res. 2(2): 113-124

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