Alternative names:
basic FGF, HBGF-2

Data and images for Human FGF-2

Figures

Figure 1

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In vitro generation of primary mouse neurospheres.
A single-cell suspension was prepared from mouse brain using the Neural Tissue Dissociation Kit (P) and the gentleMACS™ Dissociator. Isolated Prominin-1
+
cells were cultivated for 7 days in MACS NeuroMedium containing MACS NeuroBrew, Human FGF-2 (20 ng/mL), and Human EGF (20 ng/mL).

Figure 1

In vitro generation of primary mouse neurospheres.
A single-cell suspension was prepared from mouse brain using the Neural Tissue Dissociation Kit (P) and the gentleMACS™ Dissociator. Isolated Prominin-1
+
cells were cultivated for 7 days in MACS NeuroMedium containing MACS NeuroBrew, Human FGF-2 (20 ng/mL), and Human EGF (20 ng/mL).

Figure 2

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Human FGF-2 activity assay.
The biological activity of Human FGF-2, premium grade was determined by proliferation assay using 3T3 cells.

Figure 2

Human FGF-2 activity assay.
The biological activity of Human FGF-2, premium grade was determined by proliferation assay using 3T3 cells.

Figure 3

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SDS-PAGE of Human FGF-2, premium grade
under reduced (R) and non-reduced (NR) conditions.

Figure 3

SDS-PAGE of Human FGF-2, premium grade
under reduced (R) and non-reduced (NR) conditions.

Figure 4

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Human FGF-2 biological activity.
Activity of Human FGF-2, premium grade (red bar) was compared to another commercially available product (black bar).

Figure 4

Human FGF-2 biological activity.
Activity of Human FGF-2, premium grade (red bar) was compared to another commercially available product (black bar).

Figure 5

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Mass spectrometry analysis (ESI-MS) of Human FGF-2, premium grade. The peaks correspond to the mature form of human FGF-2, both with the N-terminal proline (146 amino acids) and without the N-terminal proline (145 amino acids), respectively.

Figure 5

Mass spectrometry analysis (ESI-MS) of Human FGF-2, premium grade. The peaks correspond to the mature form of human FGF-2, both with the N-terminal proline (146 amino acids) and without the N-terminal proline (145 amino acids), respectively.

Specifications for Human FGF-2

Overview

Recombinant human FGF-2 (FGF-basic or bFGF) is used for the cultivation of undifferentiated embryonic and induced pluripotent stem cells. The basic fibroblast growth factor (FGF) also promotes the growth of a variety of cell types belonging to the mesodermal, ectodermal, and endodermal lineage. Thus, it has implications for diverse processes, such as angiogenesis, tissue repair, neuronal functions or embryogenesis.

Applications

Human FGF-2 can be used for a variety of applications, including:
  • Stimulation of proliferation and differentiation of several cell types, such as mesenchymal stromal cells, neural cells, and endothelial cells.
  • Long-term maintenance and propagation of undifferentiated embryonic and induced pluripotent stem cells.
  • Differentiation of neural cells starting from embryonic and induced pluripotent stem cell cultures.

Alternative names

basic FGF, HBGF-2

Detailed product information

Background information

Fibroblast growth factor 2 (FGF-2), also termed fibroblast growth factor basic (FGF-b) or basic FGF (bFGF), belongs to the FGF family. It functions as a wide-spectrum mitogenic, angiogenic, and neurotrophic factor and stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal, and endothelial cells. FGF-2 has been implicated in a multitude of physiological and pathological processes, including limb development, angiogenesis, wound healing, and tumor growth.

Biological activity

  • Proliferation of 3T3 cells (NIBSC 90/712)
  • research grade: ≥ 5×
    10
    5
    IU/mg
  • premium grade: ≥ 8×
    10
    5
    IU/mg
    (Typical specific activity: ≥ 1.4×
    10
    6
    IU/mg
    )
  • We measure the biological activity of each batch of MACS Premium-Grade Cytokines and state the results in the Certificate of Analysis (CoA). Based on the lot-specific activity, exact doses of active cytokine can be applied to cell culture experiments. This allows for reproducible cell culture conditions without the need for time-consuming lot-to-lot testing.

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade
cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific activities are stated in the Certificate of Analysis (www. miltenyibiotec.com/certificates).

Resources for Human FGF-2

Documents and Protocols

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for Human FGF-2

Publications

  1. Zhu, X. et al. (2015) All-Trans Retinoic Acid-Induced Deficiency of the Wnt/β-Catenin Pathway Enhances Hepatic Carcinoma Stem Cell Differentiation PLoS One 10(11): e0143255
  2. Lamanuzzi, A. et al. (2016) Role of erythropoietin in the angiogenic activity of bone marrow endothelial cells of MGUS and multiple myeloma patients Oncotarget. 7(12): 14510-14521
  3. Borchin, B. et al. (2013) Derivation and FACS-mediated purification of PAX3+/PAX7+ skeletal muscle precursors from human pluripotent stem cells. Stem Cell Reports 1: 620-631
  4. Lehnen, D. et al. (2017) IAP-Based Cell Sorting Results in Homogeneous Transplantable Dopaminergic Precursor Cells Derived from Human Pluripotent Stem Cells Stem Cell Reports 9(4): 1207-1220
  5. Koch, C. et al. (2020) Characterization of circulating breast cancer cells with tumorigenic and metastatic capacity EMBO Mol. Med. 12(9): e11908
  6. Goikuria, H. et al. (2018) Characterization of Carotid Smooth Muscle Cells during Phenotypic Transition Cells 7(3): 23
  7. Smit, D. J. et al. (2020) High Sensitivity of Circulating Tumor Cells Derived from a Colorectal Cancer Patient for Dual Inhibition with AKT and mTOR Inhibitors Cells 9(9): 2129
  8. van Tienen, F. et al. (2019) Healthy, mtDNA-mutation free mesoangioblasts from mtDNA patients qualify for autologous therapy Stem Cell Res Ther. 10(1): 405
  9. Ferreras, C. et al. (2019) Chondral Differentiation of Induced Pluripotent Stem Cells Without Progression Into the Endochondral Pathway Front Cell Dev Biol. 7: 270
  10. Sriram, K. et al. (2019) Detection and Quantification of GPCR mRNA: An Assessment and Implications of Data from High-Content Methods. ACS Omega 4(16): 17048-17059
  11. Robinson, C. J. and Gaines-Das, R. (1994)
    The international standard for basic fibroblast growth factor (FGF-2); comparison of candidate preparations by
    in vitro
    bioassays and immunoassays.
    Growth Factors 11: 9-16
  12. Barral, S. et al. (2013)
    Efficient neuronal
    in vitro
    and
    in vivo
    differentiation after immunomagnetic purification of mESC derived neuronal precursors.
    Stem Cell Res. 10(2): 133-46
  13. Golebiewska, A. et al. (2013) Side population in human glioblastoma is non-tumorigenic and characterizes brain endothelial cells. Brain 136: 1462-1475
  14. Margariti A. et al. (2012) Direct reprogramming of fibroblasts into endothelial cells capable of angiogenesis and reendothelialization in tissue-engineered vessels. Proc. Natl. Acad. Sci. U.S.A. 109: 13793-13798
  15. Barroso-delJesus, A. et al. (2011) The nodal inhibitor lefty is negatively modulated by the microRNA miR-302 in human embryonic stem cells. FASEB J. 25(5): 1497-1508
  16. Eberle, D. et al. (2011) Increased integration of transplanted CD73-positive photoreceptor precursors into adult mouse retina. Invest. Ophthalmol. Vis. Sci. 52(9): 3519

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