Untouched isolation of PBMCs from human whole blood

Successful experiments always start with efficient sample preparation. 

• The MACSprep™ PBMC Isolation Kit, human enables a safe and fast untouched isolation of human PBMCs from small whole blood samples without density gradient centrifugation. 
   

Virus-specific in vitro stimulation of T cells and other immune cells 

Our PepTivator® Peptide Pools enable efficient antigen-specific stimulation of both CD4⁺ and CD8⁺ T cells, as well as recombinant SARS-CoV-2 proteins for other immune cells. 

• SARS-CoV-2 PepTivator Peptide Pools for SARS-CoV-2-specific stimulation of T cells.
• Recombinant SARS-CoV-2 proteins are highly immunogenic and can be used for the stimulation of various immune cells.

Enrichment and sorting of antigen- and virus-specific T cells

Antigen- and virus-specific T cells are extremely rare, which makes analysis difficult. Our optimized enrichment and sorting solutions enable you to even analyze the rarest cells.

• CD137 MicroBead Kit, human and CD154 MicroBead Kit, human for the pre-enrichment of rare virus-reactive T cells based on activation marker expression.
• IFN-γ Secretion Assay – Cell Enrichment and Detection Kit (PE), human to detect and enrich viable IFNg-secreting T cells for any downstream applications. Other specificities are available.

Safe flow sorting of antigen- and virus-specific T cells

When working with contaminated or infectious material, operator safety is of the utmost importance. Our MACSQuant® Tyto® Cell Sorter allows gentle and sterile sorting of even the rarest virus-specific T cells in a closed aerosol-free cartridge system.

• The MACSQuant® Tyto® Cell Sorter for safe, gentle and sterile sorting of infectious material
• Customer stories – exciting stories from customers overcoming challenges in cell sorting 

Multiparameter flow analysis of antigen- and virus-specific T cells

Our MACS® Flow Cytometry kits and REAfinity™ Recombinant Antibodies are optimized for lowest background, highest reproducibility and most precise results. The MACSQuant Analyzer family with automated MACSExpress® Analyses modes eliminate operator variability and standardize your analysis further.

• SARS-CoV-2 T Cell Analysis Kits – true all-in-one kits for the efficient stimulation and analysis of human SARS-CoV-2-reactive T cells. Kits include all needed buffers and controls, a SARS-CoV-2 PepTivator of choice and an optimized flow panel (see table below)
• REAfinity™ Recombinant Antibodies - recombinantly engineered for higher purity, superior lot-to-lot consistency, and lowest background.
• MACSplex Cytokine Kits for a multiplexed analysis of secreted cytokines in cell culture supernatant.
• Rapid Cytokine Inspector Kits - unique for the fastest combination of surface marker and intracellular cytokine staining.
• 8-Color Immunophenotyping Kit, human for reliable identification of leukocyte subsets in one simple kit, e.g. monocytes, neutrophils, eosinophils, and T, B, and NK cell populations
• StainExpress™ Immune Cell Composition Cocktail, human – highest standardization with dried antibody cocktail for a reliable evaluation of immune cell composition from human blood material (e.g. whole blood or PBMCs) 
• MACSQuant® Analyzers - Fully automated benchtop flow cytometers for highly sensitive, multiparameter flow analyses.

Being recombinantly engineered, REAfinity recombinant Antibodies drastically reduce flow background due to a mutated binding site and provide highest purity and lot-to-lot consistency for greater reproducibility.

*For VioDye spectra see here