T cells play a pivotal role in fighting viral infections and generating long-term protective immunity after infection and vaccination.
Assessment of specificity, phenotype, and cytokine secretion of rare virus-reactive T cells can provide essential information on infection progression, prior infections or vaccination efficacy.
Successful experiments always start with efficient sample preparation.
Our PepTivator® Peptide Pools enable efficient antigen-specific stimulation of both CD4+ and CD8+ T cells, as well as recombinant SARS-CoV-2 proteins for other immune cells.
Expansion, restimulation, and analysis of multivirus-specific T cells (application note)
Expansion of antigen-specific cytokine-secreting T cells (application protocol)
Antigen- and virus-specific T cells are extremely rare, which makes analysis difficult. Our optimized enrichment and sorting solutions enable you to even analyze the rarest cells.
When working with contaminated or infectious material, operator safety is of the utmost importance. Our MACSQuant® Tyto® Cell Sorter allows gentle and sterile sorting of even the rarest virus-specific T cells in a closed aerosol-free cartridge system.
Our MACS® Flow Cytometry kits and REAfinity™ Recombinant Antibodies are optimized for lowest background, highest reproducibility and most precise results. The MACSQuant Analyzer family with automated MACSExpress® Analyses modes eliminate operator variability and standardize your analysis further.
Optimized flow panel for SARS-CoV-2 T Cell Analysis
Marker | Clone | Fluorophore |
---|---|---|
CD3 | REA613 | APC |
CD4 | REA623 | Vio® Bright B515 |
CD8 | REA734 | VioGreen™ |
CD154 | REA238 | APC-Vio® 770 |
IFN-g | REA600 | PE |
IL-2 | REA689 | PE-Vio® 615 |
TNF-a | REA656 | PE-Vio® 770 |
CD14 | REA599 | VioBlue® |
CD20 | REA780 | VioBlue |
Live/dead | Viobility™ Fixable Dye | 405/452 |
Being recombinantly engineered, REAfinity recombinant Antibodies drastically reduce flow background due to a mutated binding site and provide highest purity and lot-to-lot consistency for greater reproducibility.
*For VioDye spectra see here
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