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Our MACSPlex Mix products allow you to combine individual analytes within one application category according to your needs. This option is available for the MACSPlex Cytokine Kit, human, and the MACSPlex Cytokine Kit, mouse, but will be extended soon.
To put together your MACSPlex Mix, you need the following components:
Because each single reagent has a specific fluorescence property, the combination of analytes is only possible within one defined application category. After choosing your first analyte, compatible reagents will be visible by clicking on the product name on the product page. Human and mouse specificities cannot be mixed within a single experiment.
Overview of combinable reagents and necessary software versions to run Express Modes
|Application category||Number of analytes||Available target analytes||MACSQuantify Software version||Express Mode package|
|Human cytokines||Up to 7 analytes of choice||IL-2, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12, IL-17A, GM-CSF, IFN-α, IFN-γ, TNF-α||2.8 and higher||28.1.15443 and higher|
|Mouse cytokines||Up to 7 analytes of choice||IL-2, IL-4, IL-5, IL-10, IL-12, IL-17A, IL-23, GM-CSF, IFN-γ, TNF-α||2.8 and higher||28.1.15443 and higher|
For MACSPlex assays a flow cytometer with a blue (e.g. 488 nm) and a red (e.g. 635 nm) laser with the capability to detect FITC, PE, and APC is necessary.
ExpressModes are compatible with the MACSQuant Analyzer 10 and MACSQuant X instruments and with the MACSQuantify™ Software already installed on your MACSQuant Analyzer. For the required software version, please refer to the table above.
For all users running MACSPlex assays on a different flow cytometer, subsequent analysis can be performed on your computer. For this, you can use our Flowlogic™ Software in combination with the Beadlogic™ Add-on specifically developed to analyze multiplex assays.
Additionally, third-party software can be used for analysis.