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Human PBMC depleted of CD14 + cells were stimulated for 4 hours with LPS (10 ng/mL) and for 2 hours with R-848 (2.5 μg/mL), or left untreated. The stimulated and unstimulated cells were stained using the IL-12 Secretion Assay - Detection Kit (PE). Additionally, cells were stained with CD11c-APC. |
Stimulated sample | Unstimulated control |
IL-12 Secretion Assay – Detection Kit (PE), humanFigure 1Human PBMC depleted of CD14 + cells were stimulated for 4 hours with LPS (10 ng/mL) and for 2 hours with R-848 (2.5 μg/mL), or left untreated. The stimulated and unstimulated cells were stained using the IL-12 Secretion Assay - Detection Kit (PE). Additionally, cells were stained with CD11c-APC. | IL-12 Secretion Assay – Detection Kit (PE), humanFigure 1Human PBMC depleted of CD14 + cells were stimulated for 4 hours with LPS (10 ng/mL) and for 2 hours with R-848 (2.5 μg/mL), or left untreated. The stimulated and unstimulated cells were stained using the IL-12 Secretion Assay - Detection Kit (PE). Additionally, cells were stained with CD11c-APC. |
Human PBMC depleted of CD14 + cells were stimulated for 4 hours with LPS (10 ng/mL) and for 2 hours with R-848 (2.5 μg/mL), or left untreated. The stimulated and unstimulated cells were stained using the IL-12 Secretion Assay - Detection Kit (PE). Additionally, cells were stained with CD11c-APC. |
Stimulated sample | Unstimulated control |
IL-12 Secretion Assay – Detection Kit (PE), humanFigure 1Human PBMC depleted of CD14 + cells were stimulated for 4 hours with LPS (10 ng/mL) and for 2 hours with R-848 (2.5 μg/mL), or left untreated. The stimulated and unstimulated cells were stained using the IL-12 Secretion Assay - Detection Kit (PE). Additionally, cells were stained with CD11c-APC. | IL-12 Secretion Assay – Detection Kit (PE), humanFigure 1Human PBMC depleted of CD14 + cells were stimulated for 4 hours with LPS (10 ng/mL) and for 2 hours with R-848 (2.5 μg/mL), or left untreated. The stimulated and unstimulated cells were stained using the IL-12 Secretion Assay - Detection Kit (PE). Additionally, cells were stained with CD11c-APC. |
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