Endothelial Cell Isolation Kit, rat

Name: Endothelial Cell Isolation Kit, rat
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Endothelial Cell Isolation Kit, rat

The Endothelial Cell Isolation Kit, rat enables the enrichment of endothelial cells from adult rat hearts, muscle, fat, and neonatal brain using MACS

^®

Cell Separation technology.

The isolation is performed in a two-step procedure. The enriched endothelial cells are fully functional and can be used for various downstream applications.

Data and images
Specifications

Product data and images for Endothelial Cell Isolation Kit, rat

Figures

Figure 1

Endothelial cells were isolated from adult CD-1

^®

rat hearts by using the Endothelial Cell Isolation Kit, one LD Column with MidiMACS™ Separator, and one MS Column with MiniMACS™ Separator. The cells were fluorescently stained with Labeling Check Reagent-VioBlue

^®

and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

A:	B:
Before separation	Enriched endothelial cells
View details Endothelial Cell Isolation Kit, rat Figure 1 Endothelial cells were isolated from adult CD-1 ^® rat hearts by using the Endothelial Cell Isolation Kit, one LD Column with MidiMACS™ Separator, and one MS Column with MiniMACS™ Separator. The cells were fluorescently stained with Labeling Check Reagent-VioBlue ^® and analyzed by flow cytometry using the MACSQuant ^® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.	View details Endothelial Cell Isolation Kit, rat Figure 1 Endothelial cells were isolated from adult CD-1 ^® rat hearts by using the Endothelial Cell Isolation Kit, one LD Column with MidiMACS™ Separator, and one MS Column with MiniMACS™ Separator. The cells were fluorescently stained with Labeling Check Reagent-VioBlue ^® and analyzed by flow cytometry using the MACSQuant ^® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for Endothelial Cell Isolation Kit, rat

Overview

The Endothelial Cell Isolation Kit, rat enables the enrichment of endothelial cells from adult rat hearts, muscle, fat, and neonatal brain using MACS

^®

Cell Separation technology.

The isolation is performed in a two-step procedure. The enriched endothelial cells are fully functional and can be used for various downstream applications.

Detailed product information

Background information

Endothelial cells form the inner layer of blood vessels and play a key role in vascular development, homeostasis, and remodeling.

The Endothelial Cell Isolation Kit, rat has been designed for enrichment of endothelial cells from dissociated adult rat hearts, muscle, fat tissue, and neonatal brain.

The isolation of endothelial cells has successfully been tested using

adult rat heart in combination with the Neonatal Heart Dissociation Kit, mouse and rat (# 130-098-373)
adult rat muscle tissue in combination with the Skeletal Muscle Dissociation Kit, mouse and rat (# 130-098-305)
adult rat fat tissue in combination with the Adipose Tissue Dissociation Kit, mouse and rat (# 130-105-808)
neonatal rat brain in combination with the Neural Tissue Dissociation Kit (P) (# 130-092-628).

Columns

For the first magnetic separation (depletion): LD Columns. For the second magnetic separation (positive selection): MS Columns.

Data and images

Data and images for Endothelial Cell Isolation Kit, rat

Figures

Figure 1

Endothelial cells were isolated from adult CD-1

^®

rat hearts by using the Endothelial Cell Isolation Kit, one LD Column with MidiMACS™ Separator, and one MS Column with MiniMACS™ Separator. The cells were fluorescently stained with Labeling Check Reagent-VioBlue

^®

and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

A:	B:
Before separation	Enriched endothelial cells
View details Endothelial Cell Isolation Kit, rat Figure 1 Endothelial cells were isolated from adult CD-1 ^® rat hearts by using the Endothelial Cell Isolation Kit, one LD Column with MidiMACS™ Separator, and one MS Column with MiniMACS™ Separator. The cells were fluorescently stained with Labeling Check Reagent-VioBlue ^® and analyzed by flow cytometry using the MACSQuant ^® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.	View details Endothelial Cell Isolation Kit, rat Figure 1 Endothelial cells were isolated from adult CD-1 ^® rat hearts by using the Endothelial Cell Isolation Kit, one LD Column with MidiMACS™ Separator, and one MS Column with MiniMACS™ Separator. The cells were fluorescently stained with Labeling Check Reagent-VioBlue ^® and analyzed by flow cytometry using the MACSQuant ^® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications

Specifications for Endothelial Cell Isolation Kit, rat

Overview

The Endothelial Cell Isolation Kit, rat enables the enrichment of endothelial cells from adult rat hearts, muscle, fat, and neonatal brain using MACS

^®

Cell Separation technology.

The isolation is performed in a two-step procedure. The enriched endothelial cells are fully functional and can be used for various downstream applications.

Detailed product information

Background information

Endothelial cells form the inner layer of blood vessels and play a key role in vascular development, homeostasis, and remodeling.

The Endothelial Cell Isolation Kit, rat has been designed for enrichment of endothelial cells from dissociated adult rat hearts, muscle, fat tissue, and neonatal brain.

The isolation of endothelial cells has successfully been tested using

adult rat heart in combination with the Neonatal Heart Dissociation Kit, mouse and rat (# 130-098-373)
adult rat muscle tissue in combination with the Skeletal Muscle Dissociation Kit, mouse and rat (# 130-098-305)
adult rat fat tissue in combination with the Adipose Tissue Dissociation Kit, mouse and rat (# 130-105-808)
neonatal rat brain in combination with the Neural Tissue Dissociation Kit (P) (# 130-092-628).

Columns

For the first magnetic separation (depletion): LD Columns. For the second magnetic separation (positive selection): MS Columns.

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Endothelial Cell Isolation Kit, rat

Endothelial Cell Isolation Kit, rat

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Specifications for Endothelial Cell Isolation Kit, rat

Overview

Detailed product information

Background information

Columns

Data and images

Data and images for Endothelial Cell Isolation Kit, rat

Figures

Figure 1

Figure 1

Figure 1

Specifications

Specifications for Endothelial Cell Isolation Kit, rat

Overview

Detailed product information

Background information

Columns

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Endothelial Cell Isolation Kit, rat

6 products available | view all view less

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Endothelial Cell Isolation Kit, rat

Endothelial Cell Isolation Kit, rat

Product data and images for Endothelial Cell Isolation Kit, rat

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Figure 1