CD56 Antibody, anti-human,
REAlease
^®

Name: CD56 Antibody, anti-human, REAlease®
Availability: InStock

CD56 Antibody, anti-human,
REAlease
^®
Extended ValidationRecombinant

Clone:

REAL161

Type of antibody:

Releasable antibodies, Primary antibodies, Recombinant antibodies

Applications:

FC

Alternative names:

NCAM1, MSK39, NCAM, Leu-19, NKH-1

Flow cytometry applications forCD56 Antibody, anti-human,
REAlease
^®

APC
FITC
PE

Conjugate:

APC

Recommended dilution:

1:50

Tested:

QC tested

Products used:

130-119-685, 130-119-587, 130-122-710

Protocols:

Cell surface staining protocol using REAlease™ antibodies

Description:

Human peripheral blood mononuclear cells (PBMCs) were stained with REAlease CD56 Complexes or with the corresponding REAL Control (left images) as well as with CD3 antibodies and analyzed by flow cytometry using the MACSQuant^® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence.

Extended validation for CD56 Antibody, anti-human,
REAlease
^®

Specificity

Knockout validation

To ensure antibody specificity, the target gene is knocked out in a suitable cell line using the CRISPR/Cas9 system and the knockout is confirmed by sequencing of the target locus. The antibody is considered to bind specifically to the intended epitope if no antibody binding to the knockout cells can be detected. The antibody staining is controlled by fluorescence microscopy and/or flow cytometry.

WT

KO

Fluorescence microscopy image of CD56 knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD56-PE (REAL161, red) and counterstained with DRAQ5 (blue) as DNA stain.

Fluorescence microscopy image of CD56 knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD56-PE (REAL161, red) and counterstained with DRAQ5 (blue) as DNA stain.

Overlay histogram showing flow cytometric analysis of CD56 knockout cells. Wild type (red) and knockout cells (blue) were stained with CD56c-PE, clone (REAL161). Flow cytometry was performed with the MACSQuant

^®

Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Overlay histogram showing flow cytometric analysis of CD56 knockout cells. Wild type (red) and knockout cells (blue) were stained with CD56c-PE, clone (REAL161). Flow cytometry was performed with the MACSQuant

^®

Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for CD56 Antibody, anti-human,
REAlease
^®

Overview

Clone REAL161 is an antibody fragment derived from the full CD56 antibody molecule. It displays no binding to Fc receptors. The recombinantly engineered antibody fragments are multimerized to form the REAlease Complex to bind markers with high avidity.

Clone REAL161 recognizes the human CD56 antigen, a glycoprotein of the Ig-superfamily, also known as neural cell adhesion molecule (NCAM), which is expressed in blood on practically all resting and activated NK cells and on a minor subset of CD3

⁺

T cells. CD56 is also expressed in brain (cerebellum and cortex) and at neuromuscular junctions. Certain large granular lymphocyte (LGL) leukemias, small-cell lung carcinomas, neuronal-derived tumors, myelomas, and myeloid leukemias also express CD56.

The REAlease Kits consist of the respective fluorochrome-conjugated REAlease Complexes and the REAlease Support Kit for removal of the REAlease Complexes and optional relabeling with different fluorochrome-conjugated REAlease Complexes.

Alternative names

NCAM1, MSK39, NCAM, Leu-19, NKH-1

Detailed product information

Technical specifications

Clone	REAL161
Clonality	monoclonal
Isotype control	Control Antibody
Host	cell line
Type of antibody	Releasable antibodies, Primary antibodies, Recombinant antibodies
Species	human
Cross-reactivity	rhesus monkey ( Macaca mulatta ) , cynomolgus monkey ( Macaca fascicularis )
Antigen	CD56
Alternative names of antigen	NCAM1, MSK39, NCAM, Leu-19, NKH-1
Distribution of antigen	NK cells, T cells
RRID	AB_2751436, AB_2751418, AB_2751803, AB_2751743, AB_2784255, AB_2784254, AB_2801688, AB_2811700, AB_2801687

Resources for CD56 Antibody, anti-human,
REAlease
^®

Certificates

Please follow this

link

to search for Certificates of Analysis (CoA) by lot number.

References for CD56 Antibody, anti-human,
REAlease
^®

Publications

Carter, D. L. et al. (1999)
CD56 identifies monocytes and not natural killer cells in
Rhesus macaques
.
Cytometry 37: 41-50
Meng, J. et al. (2011) Contribution of human muscle-derived cells to skeletal muscle regeneration in dystrophic host mice. PLoS One 6(3): e17454
Stockholm, D. et al. (2011) Bistable cell fate specification as a result of stochastic fluctuations and collective spatial cell behaviour. PLoS One 5(12): e14441

Applications

Extended validation