The B Cell Expansion Kit has been developed for the activation and expansion of human B cells. The CD40-Ligand Multimer mimics a T cell-dependent activation of B cells. The B cell expansion is achieved by culturing and restimulation at day 7 and 10 of culture.

Data and images for B Cell Expansion Kit, human

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Figure 1

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The components of the CD40-Ligand Multimer Kit were pre-incubated 30 minutes at room temperature. B cells were isolated using CD19 MicroBeads and cultured (StemMACS HSC Expansion Media XF supplemented with 5% AB serum and 2 µL IL-4) at a final density or 0.15×10
6
cells per mL (low density).
On day 7 and 10 cells were harvested, counted, and restimulated. All cells were harvested, counted and analyzed on day 14. With the low density protocol a B cell expansion of up to 10-fold can be achieved.

Figure 1

The components of the CD40-Ligand Multimer Kit were pre-incubated 30 minutes at room temperature. B cells were isolated using CD19 MicroBeads and cultured (StemMACS HSC Expansion Media XF supplemented with 5% AB serum and 2 µL IL-4) at a final density or 0.15×10
6
cells per mL (low density).
On day 7 and 10 cells were harvested, counted, and restimulated. All cells were harvested, counted and analyzed on day 14. With the low density protocol a B cell expansion of up to 10-fold can be achieved.

Specifications for B Cell Expansion Kit, human

Overview

The B Cell Expansion Kit has been developed for the activation and expansion of human B cells. The CD40-Ligand Multimer mimics a T cell-dependent activation of B cells. The B cell expansion is achieved by culturing and restimulation at day 7 and 10 of culture.

Detailed product information

Background information

CD40-Ligand, also known as CD40L, CD154, TRAP, or TNFSF5 is a member of the TNF superfamily transiently expressed on the surface of activated CD4
+
T lymphocytes. It is either expressed membrane-bound or proteolytically released as a soluble form which comprises 2/3 of the extracellular domain. Its receptor CD40 is found on antigen-presenting cells such as B cells, dendritic cells, and macrophages. CD40L-CD40 interaction is important in T cell-APC (antigen-presenting cell) interaction and is e.g. involved in B cell differentiation and proliferation, isotype class-switching, and protection of B cells from apoptosis. The B Cell Expansion Kit contains the CD40-Ligand and a cross-linking antibody for multimerization to increase the biological activity of CD40-Ligand, as well as premium grade Human IL-4 and StemMACS HSC Expansion Medium XF, human.

Applications

Activation and expansion of human B cells.

Resources for B Cell Expansion Kit, human

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