TRA-1-85 (CD147) Antibody, anti-human, REAfinity™

Name: TRA-1-85 (CD147) Antibody, anti-human, REAfinity™
Availability: InStock

TRA-1-85 (CD147) Antibody, anti-human, REAfinity™Extended ValidationRecombinant

Clone:

REA476

Type of antibody:

Primary antibodies, Recombinant antibodies

Isotype:

recombinant human IgG1

Applications:

FC, MICS, IF, IHC

Alternative names:

EMMPRIN, Basigin (BSG), Neurothelin, 5F7, BSG, TCSF, OKa, CD147

Flow cytometry applications forTRA-1-85 (CD147) Antibody, anti-human, REAfinity™

APC
APC-Vio 770
Biotin
PE
PE-Vio 770
Vio Bright FITC
Vio Bright V423

Conjugate:

APC

Recommended dilution:

1:50

Remark:

Cells should be stained prior to fixation, if formaldehyde is used as a fixative.

Tested:

QC tested

Products used:

130-128-900, 130-128-833

Protocols:

Cell surface flow cytometry staining protocol (PBS/EDTA/BSA 1:50)

Description:

Human peripheral blood mononuclear cells (PBMCs) were stained with Anti-TRA-1-85 (CD147) antibodies or with the corresponding REA Control (S) antibodies (left peak). Flow cytometry was performed with the MACSQuant^® Analyzer. CD14⁺ cells were pregated for the analysis. The Tandem Signal Enhancer has been used to increase binding specificity of tandem-dye–conjugated antibodies. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandem conjugates.

MICS (MACSima Imaging Cyclic Staining) applications forTRA-1-85 (CD147) Antibody, anti-human, REAfinity™

APC
PE

Conjugate:

APC

Recommended dilution:

1:50

Fixation:

FFPE

Tested:

during development

Species tested:

human

Products used:

130-128-900, 130-128-833

Compatible applications:

IF, IHC

Protocols:

Protocol for MICS experiments

Conjugate:

APC

Recommended dilution:

1:50

Fixation:

Acetone

Tested:

during development

Species tested:

human

Products used:

130-128-900, 130-128-833

Compatible applications:

IF, IHC

Protocols:

Protocol for MICS experiments

Conjugate:

APC

Recommended dilution:

1:50

Fixation:

PFA

Tested:

during development

Species tested:

human

Products used:

130-128-900, 130-128-833

Compatible applications:

IF, IHC

Protocols:

Protocol for MICS experiments

Extended validation for TRA-1-85 (CD147) Antibody, anti-human, REAfinity™

Specificity

Knockout validation

To ensure antibody specificity, the target gene is knocked out in a suitable cell line using the CRISPR/Cas9 system and the knockout is confirmed by sequencing of the target locus. The antibody is considered to bind specifically to the intended epitope if no antibody binding to the knockout cells can be detected. The antibody staining is controlled by fluorescence microscopy and/or flow cytometry.

WT

KO

Fluorescence microscopy image of TRA-1-85 (CD147) knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with TRA-1-85 (CD147)-PE (REA476, red) and counterstained with DRAQ5 (blue) as DNA stain.

Fluorescence microscopy image of TRA-1-85 (CD147) knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with TRA-1-85 (CD147)-PE (REA476, red) and counterstained with DRAQ5 (blue) as DNA stain.

Overlay histogram showing flow cytometric analysis of CD147 knockout cells. Wild type (red) and knockout cells (blue) were stained with CD147-PE, clone (REA476). Flow cytometry was performed with the MACSQuant

^®

Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Overlay histogram showing flow cytometric analysis of CD147 knockout cells. Wild type (red) and knockout cells (blue) were stained with CD147-PE, clone (REA476). Flow cytometry was performed with the MACSQuant

^®

Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for TRA-1-85 (CD147) Antibody, anti-human, REAfinity™

Overview

Clone REA476 recognizes the human TRA-1-85 antigen, a single-pass type I membrane protein, which is also known as CD147, basigin, or extracellular matrix metalloproteinase inducer (EMMPRIN). TRA-1-85 is expressed by many cell types, including epithelial cells, endothelial cells, leukocytes, and tumor cells. It is present in vascular endothelium in non-neoplastic regions of the brain, and in tumor cells but not in proliferating blood vessels in malignant gliomas. TRA-1-85 has been implicated in a variety of physiological and pathological activities. It is best known for its ability to function as extracellular matrix metalloproteinase inducer, but has also been shown to regulate lymphocyte responsiveness, monocarboxylate transporter expression, and spermatogenesis. TRA-1-85 is often used to discern human cells from non-human cells in multiple applications including animal transplantation studies.

Additional information: Clone REA476 displays negligible binding to Fc receptors.

Alternative names

EMMPRIN, Basigin (BSG), Neurothelin, 5F7, BSG, TCSF, OKa, CD147

Detailed product information

Technical specifications

Clone	REA476
Clonality	monoclonal
Isotype	recombinant human IgG1
Isotype control	REA Control Antibody (S), human IgG1
Host	human cell line
Type of antibody	Primary antibodies, Recombinant antibodies
Species	human
Antigen	TRA-1-85 (CD147)
Alternative names of antigen	EMMPRIN, Basigin (BSG), Neurothelin, 5F7, BSG, TCSF, OKa, CD147
Molecular mass of antigen [kDa]	40
Distribution of antigen	leukocytes, endothelial cells, epithelial cells, tumor cells
Entrez Gene ID	682
RRID	AB_2905448, AB_2654232, AB_2654233, AB_2654238, AB_2654239, AB_2654240, AB_2654241, AB_2654242, AB_2654243, AB_2928936, AB_2928935, AB_2921968, AB_2921951, AB_2905449

Resources for TRA-1-85 (CD147) Antibody, anti-human, REAfinity™

Certificates

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References for TRA-1-85 (CD147) Antibody, anti-human, REAfinity™

Publications

Biswas, C. et al. (1995) The human tumor cell-derived collagenase stimulatory factor (renamed EMMPRIN) is a member of the immunoglobulin superfamily. Cancer Res. 55(2): 434-439
Williams, B. P. et al. (1988) Biochemical and genetic analysis of the OKa blood group antigen. Immunogenetics 27(5): 322-329
Yurchenko, V. et al. (2006) Dealing with the family: CD147 interactions with cyclophilins. Immunology 117(3): 301-309

Applications

Extended validation