The Neonatal Cardiac Fibroblast Isolation Kit, rat has been designed for the enrichment of cardiac fibroblasts from dissociated neonatal rat hearts. The isolation of cardiac fibroblasts has been successfully tested using neonatal rat hearts from postnatal day 0 to day 3 (P0– P3).

Cardiac fibroblasts are one of the most frequent cell types within the heart and play a critical role (i) in heart development, (ii) in maintaining normal cardiac function, as well as (iii) in cardiac remodeling during pathological conditions such as myocardial infarction. Moreover, cardiac fibroblasts are believed to be an ideal target population to be reprogrammed into cardiomyocyte-like cells for regenerative purposes. The Neonatal Cardiac Fibroblast Isolation Kit, rat has been designed for the enrichment of cardiac fibroblasts from dissociated neonatal rat hearts. The isolation of cardiac fibroblasts has been tested using neonatal rat hearts from postnatal day 0 to day 3 (P0–3). For optimal results, the Neonatal Cardiac Fibroblast Isolation Kit should be used in combination with the Neonatal Heart Dissociation Kit, mouse and rat (# 130-098-373).