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Naive CD8
+
T Cell Isolation Kit
, human
Naive CD8
+
T Cell Isolation Kit
, human
The Naive CD8
+
T Cell Isolation Kit was developed for the isolation of naive CD8
T cells were isolated from human PBMCs by using the Naive CD8
+
T Cell Isolation Kit, an LD Column and two MS Columns, a MidiMACS™ Separator and a MiniMACS™ Separator. Cells were stained with CD8-PE-Cy7, CD56-PE, CD57-PE, CD45RO-PE, CD45RA-APC, and Anti-CCR7-FITC.
T cells were isolated from human PBMCs by using the Naive CD8
+
T Cell Isolation Kit, an LD Column and two MS Columns, a MidiMACS™ Separator and a MiniMACS™ Separator. Cells were stained with CD8-PE-Cy7, CD56-PE, CD57-PE, CD45RO-PE, CD45RA-APC, and Anti-CCR7-FITC.
T cells were isolated from human PBMCs by using the Naive CD8
+
T Cell Isolation Kit, an LD Column and two MS Columns, a MidiMACS™ Separator and a MiniMACS™ Separator. Cells were stained with CD8-PE-Cy7, CD56-PE, CD57-PE, CD45RO-PE, CD45RA-APC, and Anti-CCR7-FITC.
T cells were isolated from human PBMCs by using the Naive CD8
+
T Cell Isolation Kit, an LD Column and two MS Columns, a MidiMACS™ Separator and a MiniMACS™ Separator. Cells were stained with CD8-PE-Cy7, CD56-PE, CD57-PE, CD45RO-PE, CD45RA-APC, and Anti-CCR7-FITC.
T cells were isolated from human PBMCs by using the Naive CD8
+
T Cell Isolation Kit, an LD Column and two MS Columns, a MidiMACS™ Separator and a MiniMACS™ Separator. Cells were stained with CD8-PE-Cy7, CD56-PE, CD57-PE, CD45RO-PE, CD45RA-APC, and Anti-CCR7-FITC.
T cells were isolated from human PBMCs by using the Naive CD8
+
T Cell Isolation Kit, an LD Column and two MS Columns, a MidiMACS™ Separator and a MiniMACS™ Separator. Cells were stained with CD8-PE-Cy7, CD56-PE, CD57-PE, CD45RO-PE, CD45RA-APC, and Anti-CCR7-FITC.
T cells were isolated from human PBMCs by using the Naive CD8
+
T Cell Isolation Kit, an LD Column and two MS Columns, a MidiMACS™ Separator and a MiniMACS™ Separator. Cells were stained with CD8-PE-Cy7, CD56-PE, CD57-PE, CD45RO-PE, CD45RA-APC, and Anti-CCR7-FITC.
T Cell Isolation Kit was developed for the isolation of naive CD8
+
T cells from human PBMCs in a two-step procedure.
Detailed product information
Detailed separation procedure
The isolation of naive CD8
+
T cells is performed in a two-step procedure. First, naive T cells are enriched by depletion of non-naive T cells and NK cells, which are indirectly magnetically labeled with a cocktail of biotin-conjugated antibodies and Anti-Biotin MicroBeads. In a second step, the enriched naive T cells are labeled with CD8 MicroBeads for subsequent positive selection of the CD8
+
naive T cells.
Downstream applications
Naive CD8
+
T cells isolated with the Naive CD8
+
T Cell Isolation Kit can be used for functional T cell studies, e.g., studies on signal transduction or cytokine expression after activation of the naive CD8
+
T cells.
Columns
For the first magnetic separation (depletion): LD or autoMACS
®
Columns. For the second magnetic separation (positive selection): MS or autoMACS Columns.
T cells were isolated from human PBMCs by using the Naive CD8
+
T Cell Isolation Kit, an LD Column and two MS Columns, a MidiMACS™ Separator and a MiniMACS™ Separator. Cells were stained with CD8-PE-Cy7, CD56-PE, CD57-PE, CD45RO-PE, CD45RA-APC, and Anti-CCR7-FITC.
T cells were isolated from human PBMCs by using the Naive CD8
+
T Cell Isolation Kit, an LD Column and two MS Columns, a MidiMACS™ Separator and a MiniMACS™ Separator. Cells were stained with CD8-PE-Cy7, CD56-PE, CD57-PE, CD45RO-PE, CD45RA-APC, and Anti-CCR7-FITC.
T cells were isolated from human PBMCs by using the Naive CD8
+
T Cell Isolation Kit, an LD Column and two MS Columns, a MidiMACS™ Separator and a MiniMACS™ Separator. Cells were stained with CD8-PE-Cy7, CD56-PE, CD57-PE, CD45RO-PE, CD45RA-APC, and Anti-CCR7-FITC.
T cells were isolated from human PBMCs by using the Naive CD8
+
T Cell Isolation Kit, an LD Column and two MS Columns, a MidiMACS™ Separator and a MiniMACS™ Separator. Cells were stained with CD8-PE-Cy7, CD56-PE, CD57-PE, CD45RO-PE, CD45RA-APC, and Anti-CCR7-FITC.
T cells were isolated from human PBMCs by using the Naive CD8
+
T Cell Isolation Kit, an LD Column and two MS Columns, a MidiMACS™ Separator and a MiniMACS™ Separator. Cells were stained with CD8-PE-Cy7, CD56-PE, CD57-PE, CD45RO-PE, CD45RA-APC, and Anti-CCR7-FITC.
T cells were isolated from human PBMCs by using the Naive CD8
+
T Cell Isolation Kit, an LD Column and two MS Columns, a MidiMACS™ Separator and a MiniMACS™ Separator. Cells were stained with CD8-PE-Cy7, CD56-PE, CD57-PE, CD45RO-PE, CD45RA-APC, and Anti-CCR7-FITC.
T cells were isolated from human PBMCs by using the Naive CD8
+
T Cell Isolation Kit, an LD Column and two MS Columns, a MidiMACS™ Separator and a MiniMACS™ Separator. Cells were stained with CD8-PE-Cy7, CD56-PE, CD57-PE, CD45RO-PE, CD45RA-APC, and Anti-CCR7-FITC.
T Cell Isolation Kit was developed for the isolation of naive CD8
+
T cells from human PBMCs in a two-step procedure.
Detailed product information
Detailed separation procedure
The isolation of naive CD8
+
T cells is performed in a two-step procedure. First, naive T cells are enriched by depletion of non-naive T cells and NK cells, which are indirectly magnetically labeled with a cocktail of biotin-conjugated antibodies and Anti-Biotin MicroBeads. In a second step, the enriched naive T cells are labeled with CD8 MicroBeads for subsequent positive selection of the CD8
+
naive T cells.
Downstream applications
Naive CD8
+
T cells isolated with the Naive CD8
+
T Cell Isolation Kit can be used for functional T cell studies, e.g., studies on signal transduction or cytokine expression after activation of the naive CD8
+
T cells.
Columns
For the first magnetic separation (depletion): LD or autoMACS
®
Columns. For the second magnetic separation (positive selection): MS or autoMACS Columns.
