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Untouched NK cells were isolated from 8 mL of human EDTA-anticoagulated whole blood using the MACSxpress ® Whole Blood NK Cell Isolation Kit, a MACSmix™ Tube Rotator, and a MACSxpress Separator. The isolated cells were fluorescently stained with CD45-VioBlue, CD3-FITC, and CD56-PE and analyzed by flow cytometry using the MACSQuant ® Analyzer 10. Cell debris, non-leukocytes, and dead cells were excluded from the analysis based on CD45 expression, scatter signals, and propidium iodide fluorescence. |
Before separation | After separation |
MACSxpress ® Whole Blood NK Cell Isolation Kit | MACSxpress ® Whole Blood NK Cell Isolation Kit |
Untouched NK cells were isolated from 8 mL of human EDTA-anticoagulated whole blood using the MACSxpress ® Whole Blood NK Cell Isolation Kit, a MACSmix™ Tube Rotator, and a MACSxpress Separator. The isolated cells were fluorescently stained with CD45-VioBlue, CD3-FITC, and CD56-PE and analyzed by flow cytometry using the MACSQuant ® Analyzer 10. Cell debris, non-leukocytes, and dead cells were excluded from the analysis based on CD45 expression, scatter signals, and propidium iodide fluorescence. |
Before separation | After separation |
MACSxpress ® Whole Blood NK Cell Isolation Kit | MACSxpress ® Whole Blood NK Cell Isolation Kit |
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