Optics | |
| Laser excitation | Spatially separated: 405 nm, 40 mW 488 nm, 30 mW 640 nm, 20 mW |
| Emission detectors | FSC: 488/10 nm SSC: 488/10 nm V1: 450/50 nm V2: 525/50 nm B1: 525/50 nm B2: 585/40 nm B3: 655–730 nm B4: 750 nm LP R1: 655–730 nm R2: 750 nm LP |
| Fluorescence sensitivity and resolution | MESFs (CV <5%): FITC <200 PE <100 APC <150 |
| Flow cell dimensions | 200×250 μm |
| Fluorescence detectors | Optimized with spectrally matched PMTs for all channels |
Fluidics | |
| Minimal uptake volume1 | 1 μL (20 μL recommended for volumetric counting applications) |
| Sample flow rate | 25–100 µL/min plus automated flow rate to maintain 500, 1000, or 2000 events/seconds |
| Measurement speed2,3 | <15 minutes for 96-well plate (5 µL per well) |
| Sample uptake | 1–5,000 µL |
| Maximal event rate | Up to 15,000 events/second |
Technical specifications | |
| Footprint | 385.5 × 284.5 mm (15.18 × 11.2 in) |
| Footprint with Orbital Shaker | 386 × 350 mm (15.19 × 13.78 in) |
| Size (width × depth) Height (adjustable touchscreen) | 669 × 400 mm (26.34 × 15.75 in) 394–553.5 mm (15.51–21.79 in) |
| Size with Orbital Shaker (width × depth) Height with Orbital Shaker (adjustable touchscreen) | 814 × 474 mm (32.05 × 8.66 in) 427–586.5 mm (16.81–23.09 in) |
| Weight | 50 kg (110 lbs) |
| Monitor | 15.6" LCD touchscreen |
| Power requirements | 100–240 V~, 50/60 Hz |
| Power consumption (standard use) | 450 W |
| RAM | 8 GB DDR4 (SO-DIMM) |
| Mass storage | 500 GB SSD |
| Ports | 4× USB 2.0 ports, 6× USB 3.0 ports (2 at display), 2× DisplayPort, 2× LAN, DVI, RE-232, Audio |
| Emission sound pressure level at workstation | <61 dB(A) |
Performance | |
| Sample carry-over2,4 | 0.1% |
| Fluorescence performance | 5-decade logarithmic scale (10-2 to 103), displayed in lin, log, or hlog scales |
Data management | |
| Operating system | Embedded operating system |
| Measurement parameters | Area, width, height for all parameters, with time and volume |
| Signal processing | >18 bit dynamic range in area with 32 bit floating point signal processing |
| Compensation | Automated, or manual with 8×8 matrix, during or after acquisition |
| Data files | .mqd (proprietary file format), .fcs (2.0, 3.0, 3.1 compatible) |
| 1 At every uptake, an additional excess volume is aspirated by the instrument. The excess volumes are calibration- and process-dependent, and do not exceed 15 µL. 2 Referred value indicates the average of multiple experiments and can differ for individual sample materials. 3 The measurement speed is determined by measuring the time between the movement of the robotic arm into the first measured well, and its movement out of the last measured well. The measurements were carried out at high flow rate in fast mode. 4 For carry-over, full 96-well plates were loaded with 200 μL/well of PBMC suspension at a nominal concentration of 5,000 cells/μL in every other well ("SRC-wells”). Alternating wells are loaded with an equal volume of MACSQuant® Running Buffer ("CO-wells”). The uptake volume was set to 50 μL at medium flowrate. Plate shaking was set to 1200 rpm and a singlet gate was used to determine singlet counts in originating wells as well as in carry-over wells. The carry-over is defined by sum (CO-singletcount)/sum(SRC-singletcount) ×100%. Results for 384-well plates do not differ significantly from those measured in 96-well plates. | |
Brochures/posters
Scientific posters
- REAlease® Technology: controlled release of antibody-fluorochrome conjugates for maximal flexibility in flow sorting applications
- Tumor-infiltrating T cells: Complete workflows allow faster and improved flow cytometric analysis of syngeneic mouse tumors
App notes/customer reports
- A complete macrophage suppression assay workflow making exclusive use of Miltenyi Biotec products
- Measurement assurance and harmonization using the MACSQuant® Analyzer in cell counting applications
- High-throughput flow cytometric screening of a kinase inhibitor library for apoptosis induction
- Maximize the efficiency of CAR T cell functionality assessment via flow cytometry
- High-throughput, multiplexed cytokine detection in mouse MLR
- Calcium flux assay in mixed populations using kinetic flow cytometry
- Automated FRET based high-throughputscreening with MACSQuant® X flow cytometer
- Simultaneous detection of multiple fluorescent proteins using a high-throughput flow cytometer
- High-throughput ToxTracker® ACE genotoxicity assay on the MACSQuant® X flow cytometer
- Reliable, hands-free, and high-throughput cell viability assays on the MACSQuant® Flow Cytometer
- MACSQuant® X Analyzer facilitates Ca2+ flux assays
Other downloads
Harmonizing the world is just an instrument setting away with MACSQuantify™ Software
- Easy assay transfer from one instrument to another for comparable, standardized results and multi-instrument alignment during inter-lab collaborations
- Express modes with predefined experiment settings as well as acquisition and analysis templates
- Automated fluorescence compensation
- Compliance with 21 CFR Part 11 for data submission to regulatory agencies
- Audit trail, analysis reports with e-signature, and user management system according to 21 CFR Part 11
Support at your fingertips
- MACSQuant Live Support for real-time assistance from our experts
- Technical and field application support for assay design, custom automation, and express mode development
- Training at Miltenyi University
- Comprehensive service options provided by our globally distributed field service teams
MACSQuant® X Flow Cytometer
Reliable high-throughput flow cytometry
Order no.: 130-105-100. For research use only.
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