Clone:
REA360
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IF, IHC
Alternative names:
ITGA3, MSK18, FRP-2, GAP-B3, GAPB3, ILNEB, VCA-2, VL3A, VLA-3α

Extended validation for CD49c Antibody, anti-human, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA360
REAL349++
C3 II1++
17c6+
ASC-1+
P1B5++
Cells were incubated with an excess of purified unconjugated CD49c (REA360) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD49c. Hela cells were stained with CD49c antibodies and with a suitable counterstaining. As a control, CD49c antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD49c. Hela cells were stained with CD49c antibodies and with a suitable counterstaining. As a control, CD49c antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD49c. Hela cells were stained with CD49c antibodies and with a suitable counterstaining. As a control, CD49c antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD49c. Hela cells were stained with CD49c antibodies and with a suitable counterstaining. As a control, CD49c antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD49c. Hela cells were stained with CD49c antibodies and with a suitable counterstaining. As a control, CD49c antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD49c (REA360). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD49c (REA360). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD49c (REA360). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD49c Antibody, anti-human, REAfinity™

Overview

Clone REA360 recognizes the human CD49c antigen, a single-pass type I membrane protein also known as integrin α-3 (ITGA3) or VLA-3α. CD49c forms a heterodimer with integrin β1 (α3β1, CD49c/CD29, VLA-3) and plays a role in neural migration and corticogenesis, acted upon by such factors as netrin-1 and reelin. It is expressed by many types of adhesion cells, such as endothelial cells, epithelial cells, and dermal fibroblasts. Weak expression has been reported on leukocytes. CD49c/CD29 plays a role in cell-cell and cell-matrix adhesion through binding fibronectin, laminin, collagen, epiligrin, thrombospondin, and CSPG4.
Additional information: Clone REA360 displays negligible binding to Fc receptors.

Alternative names

ITGA3, MSK18, FRP-2, GAP-B3, GAPB3, ILNEB, VCA-2, VL3A, VLA-3α

Detailed product information

Technical specifications

CloneREA360
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hosthuman cell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenCD49c
Alternative names of antigenITGA3, MSK18, FRP-2, GAP-B3, GAPB3, ILNEB, VCA-2, VL3A, VLA-3α
Molecular mass of antigen [kDa]113(sum of molecular weights of subunits)
Distribution of antigenepithelial cells, fibroblasts, leukocytes
Entrez Gene ID3675
RRIDAB_2751538, AB_2658479, AB_2658480, AB_2658483, AB_2658484, AB_2658485, AB_2658486, AB_2658487, AB_2658488, AB_2658489, AB_2658490, AB_2658491, AB_2658492, AB_2658493, AB_2658494, AB_2751548

Resources for CD49c Antibody, anti-human, REAfinity™

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for CD49c Antibody, anti-human, REAfinity™

Publications

  1. Carter, W. G. et al. (1991) Epiligrin, a new cell adhesion ligand for integrin alpha 3 beta 1 in epithelial basement membranes. Cell 65(4): 599-610
  2. Takada, Y. et al. (1991) Molecular cloning and expression of the cDNA for alpha 3 subunit of human alpha 3 beta 1 (VLA-3), an integrin receptor for fibronectin, laminin, and collagen. J. Cell Biol. 115(1): 257-266
  3. de Melker, A. A. et al. (1997) The A and B variants of the alpha 3 integrin subunit: tissue distribution and functional characterization. Lab. Invest. 76(4): 547-563

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