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The scientific community is working at full speed to fight severe acute respiratory syndrome coronavirus 2 (SARS‑CoV‑2). Miltenyi Biotec is committed to assisting those researchers in getting to the bottom of SARS‑CoV‑2.
Our newly developed SARS-CoV-2 PepTivator Peptide Pools can be used to load antigen-presenting cells and to stimulate SARS-CoV-2–specific T cells. The T cells can then be detected and/or isolated for further research. We provide four different peptide pools, covering the sequence of three major structural SARS-CoV-2 proteins.
Please note that data are derived from a single experiment and do not include multiple donors. Thus, the results should be considered as exemplary only and not as representative. Results may vary dependent on donor, time since infection, and used assays.
The surface or spike glycoprotein (S) plays a critical role in coronavirus infection as it is crucial for recognition and fusion of the virus with the host cell membrane. Two different functional domains of the S protein are responsible for these individual processes: The S1 domain contains the surface binding site to the host cell receptor, the ACE2 receptor, whereas membrane fusion is mediated via the S2 domain. Furthermore, cleavage at the boundary of the S1 and S2 domains (aa residues 685 and 686) via cellular proteases primes the protein for entry into the host cell. Upon fusion of the viral envelope and the host cell membrane, the viral genome enters the host cell and viral replication starts.
The PepTivator SARS‑CoV‑2 Prot_S covers the predicted immunodominant sequence domains of the SARS‑CoV‑2 S protein (GenBank MN908947.3, Protein QHD43416.1).
The PepTivator SARS‑CoV‑2 Prot_S1 covers the N-terminal S1 domain as it contains the amino acid sequence 1–692 of the S protein (GenBank MN908947.3, Protein QHD43416.1).
Prot_N stands for nucleocapsid phosphoprotein (N). The N protein has multiple functions in the coronavirus replication cycle. Its primary function is viral genome packaging. Furthermore, it is involved in virus transcription and interacts with the viral membrane during virion assembly.
The N protein has been suggested as a possible target for vaccine development (PMID: 32106567).
The PepTivator SARS‑CoV‑2 Prot_N covers the complete sequence of the SARS‑CoV‑2 N protein (GenBank MN908947.3, Protein QHD43423.2).
Prot_M stands for membrane glycoprotein (M). The M protein is the most abundant structural protein in the coronavirus envelope and thus defines viral morphogenesis. Additionally, the M protein is crucial for efficient virion assembly and involved in viral budding.
The M protein has been suggested as a possible target for vaccine development (PMID: 16423399).
The PepTivator SARS‑CoV‑2 Prot_M covers the complete sequence of the SARS‑CoV‑2 M protein (GenBank MN908947.3, Protein QHD43419.1).
All PepTivator SARS‑CoV‑2 products are available in two sizes (6 nmol and 60 nmol per peptide per vial) in research-grade quality.
PepTivator® Peptide Pools consist of short 15-mer peptides with 11-amino-acid overlaps, covering the amino acid sequence of a particular virus-, tumor-, or auto-antigen.
They bind to MHC class I as well as MHC class II complexes and thus are suitable for the antigen-specific stimulation of CD4+ and CD8+ T cells. Products containing 6 nmol per peptide are sufficient to stimulate up to 10⁸ cells and products containing 60 nmol per peptide are sufficient to stimulate up to 10⁹ cells.
Virus-specific PepTivator Peptide Pools can be used:
More references for PepTivator Peptide Pools can be found in our reference list available for download.
Find a complete workflow to activate, isolate, and detect virus-specific T cells here.
If you are interested in the SARS‑CoV‑2 PepTivator Peptide Pools, please fill in the contact form below and we will come back to you:
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