Immunofluorescence analysis of a mouse olfactory bulb cryosection fixed with 4% PFA. The section was stained using Tyrosine Hydroxylase Antibody, anti-human/mouse/rat, APC (green) for 10 min at room temperature. Nuclei were visualized with Hoechst™ (blue).Scale bar: 30 µm.

3D-immunofluorescence analysis of a mouse brain hemisphere fixed with 4% PFA and subjected to whole-mount immunostaining with Tyrosine Hydroxylase Antibody, anti-human/mouse, Vio R667 (red). The sample was processed and optically cleared using the MACS^® Clearing Kit and imaged with an UltraMicroscope II light sheet instrument. Sample detail image of substantia nigra (A), overview image (B), 3D-rendered maximum intensity projection (C) and 3D-rendered video. Scale bars: 100 µm (A), 500 µm (B), 80 µm (C).