Name: StraightFrom® Whole Blood CD66b MicroBeads, human
Availability: InStock

Product data and images for StraightFrom
^®
Whole Blood CD66b MicroBeads
, human

Figures

Figure 1

Separation of a whole blood sample using the StraightFrom

^®

Whole Blood CD66b MicroBeads and the MultiMACS™ Cell24 Separator Plus with the Single-Column Adapter and Whole Blood Columns without washing step after labeling. Cells were fluorescently stained with CD15‑FITC, CD66b-PE, as well as CD45‑VioBlue

^®

and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Cells were triggered via CD45-VioBlue, cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Before separation

After separation

Figure 1

Separation of a whole blood sample using the StraightFrom

^®

and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Cells were triggered via CD45-VioBlue, cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Figure 1

Separation of a whole blood sample using the StraightFrom

^®

and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Cells were triggered via CD45-VioBlue, cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for StraightFrom
^®
Whole Blood CD66b MicroBeads
, human

Overview

StraightFrom

^®

Whole Blood CD66b MicroBeads were developed for rapid positive selection of CD66b

⁺

cells directly from human whole blood. No sample preparation is required, including density gradient centrifugation or erythrocyte lysis.

Detailed product information

Background information

StraightFrom

^®

Whole Blood CD66b MicroBeads are developed for the positive selection of CD66b

⁺

cells directly from anticoagulated whole blood by using the autoMACS

^®

Pro Separator, the MultiMACS™ Cell24 Separator Plus, or the Whole Blood Column Kit. No sample preparation is required, including density gradient centrifugation or erythrocyte lysis. CD66b is a 95–100 kDa glycosylphosphatidylinositol (GPI)-linked protein also known as CD67, CGM6, and NCA-95. It is expressed on neutrophils and eosinophils but not on basophils and lymphocytes.

Detailed separation procedure

Anticoagulated whole blood samples of 0.25–15 mL are labeled with StraightFrom

^®

Whole Blood CD66b MicroBeads to be subsequently separated with the autoMACS

^®

Pro Separator, the MultiMACS™ Cell24 Separator Plus, or using the Whole Blood Column Kit.

Columns

autoMACS

^®

Columns or Whole Blood Columns.

Resources for StraightFrom
^®
Whole Blood CD66b MicroBeads
, human

Documents and Protocols

Brochures/posters

StraightFrom® MicroBeads - Cell isolation directly from blood products

Data and images

Data and images for StraightFrom
^®
Whole Blood CD66b MicroBeads
, human

Figures

Figure 1

Separation of a whole blood sample using the StraightFrom

^®

and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Cells were triggered via CD45-VioBlue, cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Before separation

After separation

Figure 1

Separation of a whole blood sample using the StraightFrom

^®

and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Cells were triggered via CD45-VioBlue, cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Figure 1

Separation of a whole blood sample using the StraightFrom

^®

and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Cells were triggered via CD45-VioBlue, cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications

Specifications for StraightFrom
^®
Whole Blood CD66b MicroBeads
, human

Overview

StraightFrom

^®

Whole Blood CD66b MicroBeads were developed for rapid positive selection of CD66b

⁺

cells directly from human whole blood. No sample preparation is required, including density gradient centrifugation or erythrocyte lysis.

Detailed product information

Background information

StraightFrom

^®

Whole Blood CD66b MicroBeads are developed for the positive selection of CD66b

⁺

cells directly from anticoagulated whole blood by using the autoMACS

^®

Detailed separation procedure

Anticoagulated whole blood samples of 0.25–15 mL are labeled with StraightFrom

^®

Whole Blood CD66b MicroBeads to be subsequently separated with the autoMACS

^®

Pro Separator, the MultiMACS™ Cell24 Separator Plus, or using the Whole Blood Column Kit.

Columns

autoMACS

^®

Columns or Whole Blood Columns.

Resources

Resources for StraightFrom
^®
Whole Blood CD66b MicroBeads
, human

Documents and Protocols

Brochures/posters

StraightFrom® MicroBeads - Cell isolation directly from blood products

Video

How to isolate immune cells directly from blood

Follow this fast and easy protocol for magnetic isolation of immune cells directly from blood or blood products without the need for density gradient centrifugation.

StraightFrom® Whole Blood CD66b MicroBeads, human

StraightFrom® Whole Blood CD66b MicroBeads, human

Product data and images for StraightFrom® Whole Blood CD66b MicroBeads, human

Figures

Figure 1