StraightFrom® Whole Blood CD45 MicroBeads

StraightFrom
®
Whole Blood CD45 MicroBeads were developed for rapid positive selection of CD45
+
cells directly from whole blood or bone marrow, thus minimizing hands-on time and maximizing yield of target cells. No sample preparation is required, including density gradient centrifugation and erythrocyte lysis.

Data and images for
StraightFrom
®
Whole Blood CD45 MicroBeads

Figures

Figure 1

View details
Leukocytes were separated from whole blood using StraightFrom
®
Whole Blood CD45 MicroBeads and the autoMACS
®
Separator with program Posseld2. Cells were fluorescently stained with CD45-FITC and CD235a (Glycophorin A)-PE.

Figure 1

Leukocytes were separated from whole blood using StraightFrom
®
Whole Blood CD45 MicroBeads and the autoMACS
®
Separator with program Posseld2. Cells were fluorescently stained with CD45-FITC and CD235a (Glycophorin A)-PE.

Specifications for
StraightFrom
®
Whole Blood CD45 MicroBeads

Overview

StraightFrom
®
Whole Blood CD45 MicroBeads were developed for rapid positive selection of CD45
+
cells directly from whole blood or bone marrow, thus minimizing hands-on time and maximizing yield of target cells. No sample preparation is required, including density gradient centrifugation and erythrocyte lysis.

Detailed product information

Background information

The CD45 antigen is expressed on all cells of hematopoietic origin except erythrocytes, platelets, and their precursor cells.

Detailed separation procedure

Anticoagulated cell samples of 0.25–15 mL are labeled with StraightFrom
®
Whole Blood CD45 MicroBeads and subsequently separated with the autoMACS
®
Pro Separator, the MultiMACS™ Cell24 Separator Plus, or using the Whole Blood Column Kit. StraightFrom Whole Blood MicroBeads in combination with the autoMACS Separators allow standardization of the cell separation procedure and safe handling of hazardous samples.

Downstream applications

The purified CD45
+
cells are well suited for flow cytometric, functional, or molecular analysis including chimerism analysis after allogeneic stem cell transplantation
1
.

Columns

autoMACS
®
Columns or Whole Blood Columns.

Resources for
StraightFrom
®
Whole Blood CD45 MicroBeads

Documents and Protocols

Brochures/posters

StraightFrom® MicroBeads - Cell isolation directly from blood products

References for
StraightFrom
®
Whole Blood CD45 MicroBeads

Publications

  1. Koehl, U. et al. (2003) Quantitative analysis of chimerism after allogeneic stem cell transplantation by PCR amplification of microsatellite markers and capillary electrophoresis with fluorescence detection: the Frankfurt experience. Leukemia 17: 232-236
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How to isolate immune cells directly from blood

Follow this fast and easy protocol for magnetic isolation of immune cells directly from blood or blood products without the need for density gradient centrifugation.

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