PepTivator® Melan-A/MART-1, human

PepTivator
®
Melan-A/MART-1 is a peptide pool that consists mainly of 15-mer peptides with 11–amino acid (aa) overlap, covering the complete sequence of the human MelanA/MART-1 protein (UniProt ID: Q16655).
PepTivator peptide pools have been developed for the efficient
in vitro
stimulation of antigen–specific CD4
+
and CD8
+
T cells as peptides of 15-aa length with 11-aa overlap represent an optimized solution for stimulating both CD4
+
and CD8
+
T cells in various applications.
1
Quantitative,

Specifications for
PepTivator
®
Melan-A/MART-1
, human

Overview

PepTivator
®
Melan-A/MART-1 is a peptide pool that consists mainly of 15-mer peptides with 11–amino acid (aa) overlap, covering the complete sequence of the human MelanA/MART-1 protein (UniProt ID: Q16655).
PepTivator peptide pools have been developed for the efficient
in vitro
stimulation of antigen–specific CD4
+
and CD8
+
T cells as peptides of 15-aa length with 11-aa overlap represent an optimized solution for stimulating both CD4
+
and CD8
+
T cells in various applications.
1
Quantitative, phenotypical, or functional analysis of Melan-A/MART-1–specific T cell immunity can provide important information on the natural course of immune responses.

Detailed product information

Background information

Melanoma antigen recognized by T cells (MART-1) belongs to the tumor-associated antigens of the group of differentiation antigens. Its expression is restricted to normal melanocytes, but it is also present in melanoma. CD4
+
and CD8
+
T lymphocytes recognizing Melan-A/MART-1 have been identified in melanoma patients. Therefore, Melan-A/MART-1 may represent a potential target for immunotherapy of melanoma.
2

Downstream applications

The
in vitro
stimulation of Melan-A/MART-1–specific CD4
+
and CD8
+
T cells with PepTivator Melan-A/MART-1 causes the secretion of effector cytokines and the upregulation of activation markers, such as CD154 or CD137. These cytokines or activation markers then allow the detection and isolation of Melan-A/MART-1 –specific T cells.
  • Detection and analysis of Melan-A/MART-1 –specific CD4+ and CD8+ effector/memory T cells in PBMCs by MACS® Cytokine Secretion Assays, intracellular cytokine staining, or other technologies
  • Isolation of viable Melan-A/MART-1–specific CD4+ T cells with the CD154 MicroBead Kit, or of CD4+ and CD8+ T cells using the CD137 MicroBead Kit or MACS Cytokine Secretion Assay – Cell Enrichment and Detection Kits. Subsequently, cells can be expanded for generation of T cell lines.
  • Generation of Melan-A/MART-1–specific CD4+ and CD8+ effector/memory T cells from naive T cell populations for research on immunotherapy and vaccination.
  • Pulsing of antigen-presenting cells.

References for
PepTivator
®
Melan-A/MART-1
, human

Publications

  1. Kiecker, F. et al. (2004) Analysis of antigen-specific T-cell responses with synthetic peptides--what kind of peptide for which purpose? Hum. Immunol. 65: 523-536
  2. Boon, T. et al. (2006) Human T cell responses against melanoma. Annu. Rev. Immunol. 24: 175-208

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