Immunofluorescence analysis of a mouse cerebellum cryosection fixed with 4% PFA. The section was stained using Neurofilament Antibody, anti-human/mouse, APC (green) for 10 min at room temperature. Nuclei were visualized with Hoechst™ (blue).Scale bar: 30 µm.

3D-immunofluorescence analysis of an adult mouse brain hemisphere fixed with 4% PFA and subjected to whole-mount immunostaining with Neurofilament Antibody, anti-human/mouse, Vio R667 (red). The sample was processed and optically cleared using the iDISCO+ clearing (for protocol details please see herehttps://idiscodotinfo.files.wordpress.com/2015/04/whole-mount-staining-bench-protocol-methanol-dec-2016.pdf and imaged with an UltraMicroscope Blaze light sheet instrument. Imaging data was digitally post-processed. Sample detail image of cerebellum (A), overview image (B), 3D-rendered maximum intensity projection (C) and 3D-rendered video (D). Scale bars: 100 µm (A), 500 µm (B), 400 µm (C).

3D-immunofluorescence analysis of a mouse brain hemisphere fixed with 4% PFA and subjected to whole-mount immunostaining with Neurofilament Antibody, anti-human/mouse, Vio R667 (red). The sample was processed and optically cleared using the MACS^® Clearing Kit and imaged with an UltraMicroscope II light sheet instrument. Sample detail image of cerebellum (A), overview image (B), 3D-rendered maximum intensity projection (C) and 3D-rendered video (D). Scale bars: 100 µm (A), 300 µm (B), 100 µm (C).