The Naive B Cell Isolation Kit II was developed for the fast and efficient isolation of untouched naive B cells from human PBMCs.

Data and images for Naive B Cell Isolation Kit II, human

Figures

Figure 1

Untouched naive B cells were isolated from human PBMCs using the Naive B Cell Isolation Kit II, an LS Column, and a MidiMACS™ Separator. Cells were stained with a B cell marker, CD19-FITC, and anti-IgD-APC and CD27-PE to discriminate naive B cells (CD19
+
IgD
+
) from other B cells, i.e., memory B cells, activated B cells, and plasma cells (CD19
+
CD27
+
).
Unseparated fraction
View details

Figure 1

Untouched naive B cells were isolated from human PBMCs using the Naive B Cell Isolation Kit II, an LS Column, and a MidiMACS™ Separator. Cells were stained with a B cell marker, CD19-FITC, and anti-IgD-APC and CD27-PE to discriminate naive B cells (CD19
+
IgD
+
) from other B cells, i.e., memory B cells, activated B cells, and plasma cells (CD19
+
CD27
+
).
View details

Figure 1

Untouched naive B cells were isolated from human PBMCs using the Naive B Cell Isolation Kit II, an LS Column, and a MidiMACS™ Separator. Cells were stained with a B cell marker, CD19-FITC, and anti-IgD-APC and CD27-PE to discriminate naive B cells (CD19
+
IgD
+
) from other B cells, i.e., memory B cells, activated B cells, and plasma cells (CD19
+
CD27
+
).
After separation
View details

Figure 1

Untouched naive B cells were isolated from human PBMCs using the Naive B Cell Isolation Kit II, an LS Column, and a MidiMACS™ Separator. Cells were stained with a B cell marker, CD19-FITC, and anti-IgD-APC and CD27-PE to discriminate naive B cells (CD19
+
IgD
+
) from other B cells, i.e., memory B cells, activated B cells, and plasma cells (CD19
+
CD27
+
).
View details

Figure 1

Untouched naive B cells were isolated from human PBMCs using the Naive B Cell Isolation Kit II, an LS Column, and a MidiMACS™ Separator. Cells were stained with a B cell marker, CD19-FITC, and anti-IgD-APC and CD27-PE to discriminate naive B cells (CD19
+
IgD
+
) from other B cells, i.e., memory B cells, activated B cells, and plasma cells (CD19
+
CD27
+
).

Figure 2

View details
Isolation of untouched naive B cells using the Naive B Cell Isolation Kit II.

Figure 2

Isolation of untouched naive B cells using the Naive B Cell Isolation Kit II.

Specifications for Naive B Cell Isolation Kit II, human

Overview

The Naive B Cell Isolation Kit II was developed for the fast and efficient isolation of untouched naive B cells from human PBMCs.

Detailed product information

Detailed separation procedure

Non-B cells (T cells, NK cells, dendritic cells, monocytes, granulocytes, platelets, and erythroid cells) and non-naive B cells (memory B cells, activated B cells, and plasma cells) are labeled with a cocktail of biotinylated CD2, CD14, CD16, CD27, CD36, CD43, and CD235a (glycophorin A) antibodies. These cells are subsequently magnetically labeled with Anti-Biotin MicroBeads. Highly pure naive B cells are obtained by depletion of the magnetically labeled cells.

Downstream applications

Purified naive B cells can be used for studies in cell biology or molecular biology and functional assays.

Columns

MS, LS, XS, or autoMACS
®
Columns.

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