Mouse IL-17 Secretion Assay – Cell Enrichment and Detection Kit (PE)

Name: Mouse IL-17 Secretion Assay – Cell Enrichment and Detection Kit (PE)
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Mouse IL-17 Secretion Assay – Cell Enrichment and Detection Kit (PE)

The Mouse IL-17 Secretion Assay - Cell Enrichment and Detection Kit was developed for the sensitive detection as well as the enrichment of mouse IL-17A–secreting cells.

Product data and images for Mouse IL-17 Secretion Assay – Cell Enrichment and Detection Kit (PE)

Figures

Figure 1

Mouse splenocytes were stimulated for 3 hours with ionomycin (1 μg/mL) and PMA (10 ng/mL) or left untreated. Cells were enriched by using the Mouse IL-17 Secretion Assay – Cell Enrichment and Detection Kit (PE). T helper cells were counterstained by using CD4-APC. B cells and dead cells were excluded from the analysis.

Stimulated sample

Before enrichment	After enrichment
View details Mouse IL-17 Secretion Assay – Cell Enrichment and Detection Kit (PE) Figure 1 Mouse splenocytes were stimulated for 3 hours with ionomycin (1 μg/mL) and PMA (10 ng/mL) or left untreated. Cells were enriched by using the Mouse IL-17 Secretion Assay – Cell Enrichment and Detection Kit (PE). T helper cells were counterstained by using CD4-APC. B cells and dead cells were excluded from the analysis.	View details Mouse IL-17 Secretion Assay – Cell Enrichment and Detection Kit (PE) Figure 1 Mouse splenocytes were stimulated for 3 hours with ionomycin (1 μg/mL) and PMA (10 ng/mL) or left untreated. Cells were enriched by using the Mouse IL-17 Secretion Assay – Cell Enrichment and Detection Kit (PE). T helper cells were counterstained by using CD4-APC. B cells and dead cells were excluded from the analysis.
Unstimulated control
Before enrichment	After enrichment
View details Mouse IL-17 Secretion Assay – Cell Enrichment and Detection Kit (PE) Figure 1 Mouse splenocytes were stimulated for 3 hours with ionomycin (1 μg/mL) and PMA (10 ng/mL) or left untreated. Cells were enriched by using the Mouse IL-17 Secretion Assay – Cell Enrichment and Detection Kit (PE). T helper cells were counterstained by using CD4-APC. B cells and dead cells were excluded from the analysis.	View details Mouse IL-17 Secretion Assay – Cell Enrichment and Detection Kit (PE) Figure 1 Mouse splenocytes were stimulated for 3 hours with ionomycin (1 μg/mL) and PMA (10 ng/mL) or left untreated. Cells were enriched by using the Mouse IL-17 Secretion Assay – Cell Enrichment and Detection Kit (PE). T helper cells were counterstained by using CD4-APC. B cells and dead cells were excluded from the analysis.

Specifications for Mouse IL-17 Secretion Assay – Cell Enrichment and Detection Kit (PE)

Overview

The Mouse IL-17 Secretion Assay - Cell Enrichment and Detection Kit was developed for the sensitive detection as well as the enrichment of mouse IL-17A–secreting cells.

Detailed product information

Background information

Interleukin 17 (IL-17) is a family of cytokines that play a central role in adaptive immunity as well as autoinflammatory disorders.

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Data from several mouse models suggest that IL-17 plays a key role in the host defense against certain extracellular bacterial infections.

Recently, a new mouse T helper cell subset has been identified, which is dinstinct from Th1 and Th2 cells: IL-17–producing CD4

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T helper (Th17) cells. Th17 cells preferentially produce IL-17A, IL-17F, IL-21, and IL-22. Receptors for IL-17 and IL-22 are expressed on various epithelial tissues, thus Th17 cells are crucial for the cross-talk between immune system and tissues.

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It is now established that Th17 cells are responsible for driving autoimmune inflammation.

After stimulation of mouse cells, IL-17 is not only secreted by CD4

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T cells, but also by CD8

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T cells, γδ T cells, NK cells, and granulocytes.

Columns

MS, LS, or autoMACS

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Columns.

References for Mouse IL-17 Secretion Assay – Cell Enrichment and Detection Kit (PE)

Publications

Weaver, C. T. et al. (2007) IL-17 family cytokines and the expanding diversity of effector T cell lineages. Annu. Rev. Immunol. 25: 821-852
Ouyang, W. et al. (2008) The biological functions of T helper 17 cell effector cytokines in inflammation. Immunity 28: 454-467

Data and images