MACSxpress
^®
Whole Blood CD4 T Cell Isolation Kit
, human

MACSxpress
^®
Whole Blood CD4 T Cell Isolation Kit
, human

The MACSxpress® Whole Blood CD4 T Cell Isolation Kit has been developed for the fast isolation of untouched T helper cells from freshly drawn anticoagulated whole blood without density gradient centrifugation and red blood cell lysis. Sample volumes of up to 30 mL can be processed.

Data and images
Specifications

Product data and images for MACSxpress
^®
Whole Blood CD4 T Cell Isolation Kit
, human

Figures

Figure 1

Untouched CD4

⁺

T cells were isolated from 30 mL of human EDTA-anticoagulated whole blood using the MACSxpress

^®

Whole Blood CD4 T Cell Isolation Kit, a MACSmix™ Tube Rotator, and a MACSxpress Separator. The isolated cells were fluorescently stained with CD45‑VioBlue

^®

, CD3‑FITC, and CD4-PE and analyzed by flow cytometry using the MACSQuant Analyzer

^®

10.

Cell debris, non-leukocytes, and dead cells were excluded from the analysis based on CD45 expression, scatter signals, and propidium iodide fluorescence.

Before separation

After separation

Figure 1

Untouched CD4

⁺

T cells were isolated from 30 mL of human EDTA-anticoagulated whole blood using the MACSxpress

^®

Whole Blood CD4 T Cell Isolation Kit, a MACSmix™ Tube Rotator, and a MACSxpress Separator. The isolated cells were fluorescently stained with CD45‑VioBlue

^®

, CD3‑FITC, and CD4-PE and analyzed by flow cytometry using the MACSQuant Analyzer

^®

10.

Cell debris, non-leukocytes, and dead cells were excluded from the analysis based on CD45 expression, scatter signals, and propidium iodide fluorescence.

Figure 1

Untouched CD4

⁺

T cells were isolated from 30 mL of human EDTA-anticoagulated whole blood using the MACSxpress

^®

Whole Blood CD4 T Cell Isolation Kit, a MACSmix™ Tube Rotator, and a MACSxpress Separator. The isolated cells were fluorescently stained with CD45‑VioBlue

^®

, CD3‑FITC, and CD4-PE and analyzed by flow cytometry using the MACSQuant Analyzer

^®

10.

Cell debris, non-leukocytes, and dead cells were excluded from the analysis based on CD45 expression, scatter signals, and propidium iodide fluorescence.

Specifications for MACSxpress
^®
Whole Blood CD4 T Cell Isolation Kit
, human

Overview

The MACSxpress® Whole Blood CD4 T Cell Isolation Kit has been developed for the fast isolation of untouched T helper cells from freshly drawn anticoagulated whole blood without density gradient centrifugation and red blood cell lysis. Sample volumes of up to 30 mL can be processed.

Detailed product information

Detailed separation procedure

With the new MACSxpress

^®

Technology, untouched human leukocyte subsets can be isolated from up to 30 mL of anticoagulated whole blood. Non-target cells are removed by immunomagnetic depletion using MACSxpress Beads. Simultaneously, erythrocytes are sedimented, yielding untouched target cells of high purity.

Downstream applications

CD4

⁺

T cells isolated with the MACSxpress

^®

Whole Blood CD4 T Cell Isolation Kit can be used, e.g., for phenotyping and functional assays.

Data and images

Data and images for MACSxpress
^®
Whole Blood CD4 T Cell Isolation Kit
, human

Figures

Figure 1

Untouched CD4

⁺

T cells were isolated from 30 mL of human EDTA-anticoagulated whole blood using the MACSxpress

^®

Whole Blood CD4 T Cell Isolation Kit, a MACSmix™ Tube Rotator, and a MACSxpress Separator. The isolated cells were fluorescently stained with CD45‑VioBlue

^®

, CD3‑FITC, and CD4-PE and analyzed by flow cytometry using the MACSQuant Analyzer

^®

10.

Cell debris, non-leukocytes, and dead cells were excluded from the analysis based on CD45 expression, scatter signals, and propidium iodide fluorescence.

Before separation

After separation

Figure 1

Untouched CD4

⁺

T cells were isolated from 30 mL of human EDTA-anticoagulated whole blood using the MACSxpress

^®

Whole Blood CD4 T Cell Isolation Kit, a MACSmix™ Tube Rotator, and a MACSxpress Separator. The isolated cells were fluorescently stained with CD45‑VioBlue

^®

, CD3‑FITC, and CD4-PE and analyzed by flow cytometry using the MACSQuant Analyzer

^®

10.

Cell debris, non-leukocytes, and dead cells were excluded from the analysis based on CD45 expression, scatter signals, and propidium iodide fluorescence.

Figure 1

Untouched CD4

⁺

T cells were isolated from 30 mL of human EDTA-anticoagulated whole blood using the MACSxpress

^®

Whole Blood CD4 T Cell Isolation Kit, a MACSmix™ Tube Rotator, and a MACSxpress Separator. The isolated cells were fluorescently stained with CD45‑VioBlue

^®

, CD3‑FITC, and CD4-PE and analyzed by flow cytometry using the MACSQuant Analyzer

^®

10.

Cell debris, non-leukocytes, and dead cells were excluded from the analysis based on CD45 expression, scatter signals, and propidium iodide fluorescence.

Specifications

Specifications for MACSxpress
^®
Whole Blood CD4 T Cell Isolation Kit
, human

Overview

The MACSxpress® Whole Blood CD4 T Cell Isolation Kit has been developed for the fast isolation of untouched T helper cells from freshly drawn anticoagulated whole blood without density gradient centrifugation and red blood cell lysis. Sample volumes of up to 30 mL can be processed.

Detailed product information

Detailed separation procedure

With the new MACSxpress

^®

Technology, untouched human leukocyte subsets can be isolated from up to 30 mL of anticoagulated whole blood. Non-target cells are removed by immunomagnetic depletion using MACSxpress Beads. Simultaneously, erythrocytes are sedimented, yielding untouched target cells of high purity.

Downstream applications

CD4

⁺

T cells isolated with the MACSxpress

^®

Whole Blood CD4 T Cell Isolation Kit can be used, e.g., for phenotyping and functional assays.

Related products for

MACSxpress
^®
Whole Blood CD4 T Cell Isolation Kit
, human

3 products available

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^®
Whole Blood CD4 T Cell Isolation Kit
, human

MACSxpress
^®
Whole Blood CD4 T Cell Isolation Kit
, human

Product data and images for MACSxpress
^®
Whole Blood CD4 T Cell Isolation Kit
, human

Figures

Figure 1

Figure 1

Figure 1

Specifications for MACSxpress
^®
Whole Blood CD4 T Cell Isolation Kit
, human

Overview

Detailed product information

Detailed separation procedure

Downstream applications

Data and images

Data and images for MACSxpress
^®
Whole Blood CD4 T Cell Isolation Kit
, human

Figures

Figure 1

Figure 1

Figure 1

Specifications

Specifications for MACSxpress
^®
Whole Blood CD4 T Cell Isolation Kit
, human

Overview

Detailed product information

Detailed separation procedure

Downstream applications

Related products for

MACSxpress
^®
Whole Blood CD4 T Cell Isolation Kit
, human

3 products available

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MACSxpress® Whole Blood CD4 T Cell Isolation Kit, human

MACSxpress® Whole Blood CD4 T Cell Isolation Kit, human

Product data and images for MACSxpress® Whole Blood CD4 T Cell Isolation Kit, human

Figures

Figure 1