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Human peripheral blood mononuclear cells (PBMCs) were either left unstimulated (left images) or stimulated with CD3 and CD28 antibodies in the presence of IL-2 and IL-4 for 2 days. Cells were washed, expanded in the presence of IL-2 and IL-4 for 3 days, and restimulated with 5 ng/mL phorbol 12-myristate 13-acetate (PMA), 500 ng/mL ionomycin, and 1 µg/mL brefeldin A for 5 hours. Cells were then fixed, permeabilized, and stained with Anti-LIF antibodies. Flow cytometry was performed using the MACSQuant® Analyzer. Cell debris were excluded from the analysis based on scatter signals.
Clone | REA350 |
---|---|
Clonality | monoclonal |
Isotype | recombinant human IgG1 |
Isotype control | REA Control Antibody (I), human IgG1 |
Host | human cell line |
Type of antibody | Primary antibodies, Recombinant antibodies |
Species | human |
Antigen | LIF |
Alternative names of antigen | CDF, DIA, HILDA, MLPLI, LIF, HSF III, D factor, Emfilermin |
Molecular mass of antigen [kDa] | 20 |
Distribution of antigen | stem cells, fibroblasts, T cells, macrophages, ES and iPS cells, embryonic germ cells, heart, liver, melanoma |
Entrez Gene ID | 3976 |
RRID | AB_2652644, AB_2652645, AB_2652646, AB_2652643 |
Clone | REA350 |
---|---|
Clonality | monoclonal |
Isotype | recombinant human IgG1 |
Isotype control | REA Control Antibody (I), human IgG1 |
Host | human cell line |
Type of antibody | Primary antibodies, Recombinant antibodies |
Species | human |
Antigen | LIF |
Alternative names of antigen | CDF, DIA, HILDA, MLPLI, LIF, HSF III, D factor, Emfilermin |
Molecular mass of antigen [kDa] | 20 |
Distribution of antigen | stem cells, fibroblasts, T cells, macrophages, ES and iPS cells, embryonic germ cells, heart, liver, melanoma |
Entrez Gene ID | 3976 |
RRID | AB_2652644, AB_2652645, AB_2652646, AB_2652643 |
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