IL-2 Secretion Assay – Detection Kits, human
Applications:
FC

Data and images for IL-2 Secretion Assay – Detection Kits, human

Figures

Figure 1

PBMCs of a CMV
+
donor were stimulated with CMV lysate. The responding cells were stained for coexpression of cytokines using the IL-2 Secretion Assay - Detection Kit (APC) and IFN-γ Secretion Assay - Detection Kit (PE). The plots show IFN-γ and IL-2 secretion by viable CD4
+
T cells.
A:
B:
Stimulated sample
Unstimulated control
View details

Figure 1

PBMCs of a CMV
+
donor were stimulated with CMV lysate. The responding cells were stained for coexpression of cytokines using the IL-2 Secretion Assay - Detection Kit (APC) and IFN-γ Secretion Assay - Detection Kit (PE). The plots show IFN-γ and IL-2 secretion by viable CD4
+
T cells.
View details

Figure 1

PBMCs of a CMV
+
donor were stimulated with CMV lysate. The responding cells were stained for coexpression of cytokines using the IL-2 Secretion Assay - Detection Kit (APC) and IFN-γ Secretion Assay - Detection Kit (PE). The plots show IFN-γ and IL-2 secretion by viable CD4
+
T cells.
* Percentage represents frequency among CD4+ T cells.

Specifications for IL-2 Secretion Assay – Detection Kits, human

Overview

The IL-2 Secretion Assay was developed for the sensitive detection of human IL-2-secreting cells.

Detailed product information

Background information

Interleukin-2 (IL-2) is a T cell-derived cytokine that promotes growth and differentiation of T cells and has pleiotropic effects on many other leukocytes. Upon activation, naive T helper cells and certain subsets of memory T cells rapidly secrete IL-2.

Downstream applications

The IL-2 Secretion Assay was developed for detection of IL-2-secreting T cells after antigen-specific stimulation. IL-2 Secretion Assay has been used to analyze IL-2-secreting CMV-specific CD4
+
T cells after stimulation with CMV lysate.
The IL-2 Secretion Assay - Detection Kit (APC) can also be used for analysis of cytokine coexpression and allows counterstaining of peptide-MHC tetramer-labeled cells conjugated to PE.

Resources for IL-2 Secretion Assay – Detection Kits, human

Documents and Protocols

Protocols

Two-color Cytokine Secretion Assays

Protocols

Detection of cytokine-secreting cells

Protocols

Combined staining of cytokine-secreting cells with peptide-MHC tetramers

Protocols

Detection of cytokine-secreting cells from whole blood

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for IL-2 Secretion Assay – Detection Kits, human

Publications

  1. Krichevsky, O. et al. (2017) A Tunable Diffusion-Consumption Mechanism of Cytokine Propagation Enables Plasticity in Cell-to-Cell Communication in the Immune System. Immunity 46(4): 609-620
  2. Weiss, A. et al. (2017) Destabilizing the autoinhibitory conformation of Zap70 induces up-regulation of inhibitory receptors and T cell unresponsiveness. J. Exp. Med. 214(3): 833-849

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