IL-2 Secretion Assay – Cell Enrichment and Detection Kit (PE), human

Name: IL-2 Secretion Assay – Cell Enrichment and Detection Kit (PE), human
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IL-2 Secretion Assay – Cell Enrichment and Detection Kit (PE), human

The IL-2 Secretion Assay - Cell Enrichment and Detection Kit was developed for the sensitive detection as well as the enrichment of human IL-2-secreting cells.

Product data and images for IL-2 Secretion Assay – Cell Enrichment and Detection Kit (PE), human

Figures

Figure 1

PBMCs from a CMV

⁺

donor were stimulated for 16 hours with CMV lysate. The responding cells were stained and isolated according to secretion of IL-2 using the IL-2 Secretion Assay - Cell Enrichment and Detection Kit.

In the stimulated sample, 2047 IL-2-secreting CD4

⁺

T cells were enriched per 10

⁶

CD4

⁺

T cells using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, only one IL-2-secreting CD4

⁺

T cell was detected per 10

⁶

CD4

⁺

T cells after enrichment.

Stimulated sample

Before enrichment	After enrichment
View details IL-2 Secretion Assay – Cell Enrichment and Detection Kit (PE), human Figure 1 PBMCs from a CMV ⁺ donor were stimulated for 16 hours with CMV lysate. The responding cells were stained and isolated according to secretion of IL-2 using the IL-2 Secretion Assay - Cell Enrichment and Detection Kit. In the stimulated sample, 2047 IL-2-secreting CD4 ⁺ T cells were enriched per 10 ⁶ CD4 ⁺ T cells using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, only one IL-2-secreting CD4 ⁺ T cell was detected per 10 ⁶ CD4 ⁺ T cells after enrichment.	View details IL-2 Secretion Assay – Cell Enrichment and Detection Kit (PE), human Figure 1 PBMCs from a CMV ⁺ donor were stimulated for 16 hours with CMV lysate. The responding cells were stained and isolated according to secretion of IL-2 using the IL-2 Secretion Assay - Cell Enrichment and Detection Kit. In the stimulated sample, 2047 IL-2-secreting CD4 ⁺ T cells were enriched per 10 ⁶ CD4 ⁺ T cells using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, only one IL-2-secreting CD4 ⁺ T cell was detected per 10 ⁶ CD4 ⁺ T cells after enrichment.
Unstimulated control

Before enrichment	After enrichment
View details IL-2 Secretion Assay – Cell Enrichment and Detection Kit (PE), human Figure 1 PBMCs from a CMV ⁺ donor were stimulated for 16 hours with CMV lysate. The responding cells were stained and isolated according to secretion of IL-2 using the IL-2 Secretion Assay - Cell Enrichment and Detection Kit. In the stimulated sample, 2047 IL-2-secreting CD4 ⁺ T cells were enriched per 10 ⁶ CD4 ⁺ T cells using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, only one IL-2-secreting CD4 ⁺ T cell was detected per 10 ⁶ CD4 ⁺ T cells after enrichment.	View details IL-2 Secretion Assay – Cell Enrichment and Detection Kit (PE), human Figure 1 PBMCs from a CMV ⁺ donor were stimulated for 16 hours with CMV lysate. The responding cells were stained and isolated according to secretion of IL-2 using the IL-2 Secretion Assay - Cell Enrichment and Detection Kit. In the stimulated sample, 2047 IL-2-secreting CD4 ⁺ T cells were enriched per 10 ⁶ CD4 ⁺ T cells using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, only one IL-2-secreting CD4 ⁺ T cell was detected per 10 ⁶ CD4 ⁺ T cells after enrichment.
* Percentage represents frequency among CD4⁺ T cells.

Specifications for IL-2 Secretion Assay – Cell Enrichment and Detection Kit (PE), human

Overview

The IL-2 Secretion Assay - Cell Enrichment and Detection Kit was developed for the sensitive detection as well as the enrichment of human IL-2-secreting cells.

Detailed product information

Background information

Interleukin-2 (IL-2) is a T cell-derived cytokine known to promote growth and differentiation of T cells and to have pleiotropic effects on many other leukocytes. Upon activation, naive T helper cells and certain subsets of memory T cells rapidly secrete IL-2.

Downstream applications

The IL-2 Secretion Assay has been developed for detection and isolation of IL-2-secreting T cells after antigen-specific stimulation. IL-2 Secretion Assay has been used to analyze IL-2-secreting CMV-specific CD4

⁺

T cells after stimulation with CMV lysate.

Columns

MS, LS, or autoMACS

^®

Columns.

Resources for IL-2 Secretion Assay – Cell Enrichment and Detection Kit (PE), human

Documents and Protocols

Application protocol

Expansion of antigen-specific cytokine-secreting T cells

Protocols

Detection and enrichment of cytokine-secreting cells from whole blood

Protocols

Detection and enrichment of cytokine-secreting cells

Data and images