Hematopoietic Lineage Labeling Cocktail, anti-mouse, Biotin

Name: Hematopoietic Lineage Labeling Cocktail, anti-mouse, Biotin
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Hematopoietic Lineage Labeling Cocktail, anti-mouse, Biotin

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Product data and images for Hematopoietic Lineage Labeling Cocktail, anti-mouse, Biotin

Figures

Figure 1

Flow cytometric analysis of mouse bone marrow cells stained with the Lineage Cell Detection Cocktail-Biotin, Anti-Biotin-FITC (A), -PE (B), or -APC (C) and CD117 antibodies. Cell debris and dead cells were excluded based on scatter signals and PI fluorescence.

A:	B:
View details Hematopoietic Lineage Labeling Cocktail, anti-mouse, Biotin Figure 1 Flow cytometric analysis of mouse bone marrow cells stained with the Lineage Cell Detection Cocktail-Biotin, Anti-Biotin-FITC (A), -PE (B), or -APC (C) and CD117 antibodies. Cell debris and dead cells were excluded based on scatter signals and PI fluorescence.	View details Hematopoietic Lineage Labeling Cocktail, anti-mouse, Biotin Figure 1 Flow cytometric analysis of mouse bone marrow cells stained with the Lineage Cell Detection Cocktail-Biotin, Anti-Biotin-FITC (A), -PE (B), or -APC (C) and CD117 antibodies. Cell debris and dead cells were excluded based on scatter signals and PI fluorescence.
C:
View details Hematopoietic Lineage Labeling Cocktail, anti-mouse, Biotin Figure 1 Flow cytometric analysis of mouse bone marrow cells stained with the Lineage Cell Detection Cocktail-Biotin, Anti-Biotin-FITC (A), -PE (B), or -APC (C) and CD117 antibodies. Cell debris and dead cells were excluded based on scatter signals and PI fluorescence.

Specifications for Hematopoietic Lineage Labeling Cocktail, anti-mouse, Biotin

Overview

The Hematopoietic Lineage Labeling Cocktail, anti-mouse, Biotin provides an easy means of detecting lineage-positive cells in mouse bone marrow and other hematopoietic tissues. Comprised of a panel of monoclonal antibodies that recognizes all mature hematopoietic lineages, the kit can be used, e.g. for discriminating (gating-out) lineage-positive cells during flow cytometry or for defining KSL cells after magnetic separation with the Anti-Sca-1 MicroBead Kit or CD117 MicroBeads. The biotinylated format provides flexibility when choosing the detection channel.

Detailed product information

Background information

Hematopoietic Lineage Labeling Cocktail, anti-mouse, Biotin containing:

CD5 Antibody, anti-mouse, Biotin (clone: 53-7.3)
CD11b Antibody, anti-human/mouse, Biotin (clone: M1/70.15.11.5)
CD45R (B220) Antibody, anti-mouse, Biotin (clone: RA3-6B2)
Ly-6B.2 Antibody, anti-mouse, Biotin, REAfinity™ (clone: REA115)
Gr-1 Antibody, anti-mouse, Biotin (clone: RB6-8C5)
Ter-119 Antibody, anti-mouse, Biotin (clone: Ter-119)

Instrument details

Flow cytometer equipped with a red (640 nm), a blue (488 nm), or a violet (405 nm) laser.

Applications

Identification and enumeration of lineage

⁺

and lineage

^–

cells from mouse bone marrow by flow cytometry.

Resources for Hematopoietic Lineage Labeling Cocktail, anti-mouse, Biotin

Documents and Protocols

App notes/customer reports

Flow cytometry analysis of mouse hematopoietic stem cells

Certificates

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References for Hematopoietic Lineage Labeling Cocktail, anti-mouse, Biotin

Publications

Halene, S. et al. (2010) Serum response factor is an essential transcription factor in megakaryocytic maturation. Blood 116: 1942-1950
Felker, P. et al. (2010) TGF-beta1 accelerates dendritic cell differentiation from common dendritic cell progenitors and directs subset specification toward conventional dendritic cells. J Immunol 185: 5326-5335
Cheng, E.-C. et al. (2009) Role for MKL1 in megakaryocytic maturation. Blood 113: 2826-2834

Data and images