Diamond CD34 Isolation Kit, human

Name: Diamond CD34 Isolation Kit, human
Availability: InStock

Diamond CD34 Isolation Kit, human

The Diamond CD34 Isolation Kit has been specifically developed for the isolation of highly pure Lin

^–

CD34

⁺

hematopoietic stem cells from peripheral blood

¹

, bone marrow

²^,3

or cord blood.

Product data and images for Diamond CD34 Isolation Kit, human

Figures

Figure 1

CD34

⁺

Lin

^–

cells were isolated from human PBMCs using the Diamond CD34 Isolation Kit, an LD Column, two MS Columns, a MidiMACS™ Separator, and a MiniMACS™ Separator. Cells were fluorescently stained with CD34-FITC, CD45-APC, and Anti-Biotin-PE and analyzed using the MACSQuant® Analyzer.

PBMCs before separation	Lineage-negative fraction
View details Diamond CD34 Isolation Kit, human Figure 1 CD34 ⁺ Lin ^– cells were isolated from human PBMCs using the Diamond CD34 Isolation Kit, an LD Column, two MS Columns, a MidiMACS™ Separator, and a MiniMACS™ Separator. Cells were fluorescently stained with CD34-FITC, CD45-APC, and Anti-Biotin-PE and analyzed using the MACSQuant® Analyzer.	View details Diamond CD34 Isolation Kit, human Figure 1 CD34 ⁺ Lin ^– cells were isolated from human PBMCs using the Diamond CD34 Isolation Kit, an LD Column, two MS Columns, a MidiMACS™ Separator, and a MiniMACS™ Separator. Cells were fluorescently stained with CD34-FITC, CD45-APC, and Anti-Biotin-PE and analyzed using the MACSQuant® Analyzer.
Isolated CD34 ⁺ Lin ^– cells
View details Diamond CD34 Isolation Kit, human Figure 1 CD34 ⁺ Lin ^– cells were isolated from human PBMCs using the Diamond CD34 Isolation Kit, an LD Column, two MS Columns, a MidiMACS™ Separator, and a MiniMACS™ Separator. Cells were fluorescently stained with CD34-FITC, CD45-APC, and Anti-Biotin-PE and analyzed using the MACSQuant® Analyzer.

Specifications for Diamond CD34 Isolation Kit, human

Overview

The Diamond CD34 Isolation Kit has been specifically developed for the isolation of highly pure Lin

^–

CD34

⁺

hematopoietic stem cells from peripheral blood

¹

, bone marrow

²^,3

or cord blood.

Detailed product information

Background information

The CD34 antigen is the most common marker for the isolation of hematopoietic stem and progenitor cells, but is also expressed on some lineage-committed cells. With the Diamond CD34 Isolation Kit, lineage-positive cells are removed in order to obtain pure, primitive CD34

⁺

HSCs.

Detailed separation procedure

The Diamond CD34 MicroBead Kit includes a lineage depletion followed by a CD34 isolation. In the first step, cells are labeled with a cocktail of biotin-conjugated antibodies against lineage-specific antigens. These cells are subsequently labeled with Anti-Biotin MicroBeads. Lineage-negative stem and progenitor cells are obtained by depletion of the magnetically labeled cells. Then CD34

⁺

cells are isolated from the lineage-negative stem and progenitor cells using CD34 MicroBeads. The resulting population obtained are CD34

⁺

Lin

^–

stem cells.

Downstream applications

Purified CD34

⁺

Lin

^-

stem cells can be used for many applications, including:

gene or miRNA expression profiling
characterization of undifferentiated stem cells and their developmental potential
stimulation of proliferation and maturation by cytokines
HSC-CFU assays
adoptive transfer and hematopoietic reconstitution

Columns

For positive selection: MS or autoMACS

^®

Columns. For depletion: LD or autoMACS Columns.

Resources for Diamond CD34 Isolation Kit, human

Documents and Protocols

Brochures/posters

Solutions for cutting-edge human hematopoietic stem cell research

Protocols

Isolation of mononuclear cells from human peripheral blood by density gradient centrifugation

Protocols

Isolation of mononuclear cells from human bone marrow aspirates by density gradient centrifugation

References for Diamond CD34 Isolation Kit, human

Publications

Wu, W.-C. et al. (2014) Circulating hematopoietic stem and progenitor cells are myeloid-biased in cancer patients. Proc. Natl. Acad. Sci. U.S.A. 111: 4221-4226
Zhang, S. et al. (2012) Matrine induces apoptosis in human acute myeloid leukemia cells via the mitochondrial pathway and Akt inactivation. PLoS One 7(10): e46853
Nair, R. R. et al. (2012) Potentiation of Nilotinib-mediated cell death in the context of the bone marrow microenvironment requires a promiscuous JAK inhibitor in CML. Leuk. Res. 36(6): 756-763

Data and images