Clone:
REA518
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IF, IHC
Alternative names:
ITGA6, CD49fB, VLA-6, VLA-6α

Extended validation for CD49f Antibody, anti-human/mouse, REAfinity™

Specificity

Knockout validation
To ensure antibody specificity, the target gene is knocked out in a suitable cell line using the CRISPR/Cas9 system and the knockout is confirmed by sequencing of the target locus. The antibody is considered to bind specifically to the intended epitope if no antibody binding to the knockout cells can be detected. The antibody staining is controlled by fluorescence microscopy and/or flow cytometry.
WT
KO
View details
Fluorescence microscopy image of CD49f knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD49f-PE (REA518, red) and counterstained with DRAQ5 (blue) as DNA stain.
View details
Fluorescence microscopy image of CD49f knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD49f-PE (REA518, red) and counterstained with DRAQ5 (blue) as DNA stain.
Fluorescence microscopy image of CD49f knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD49f-PE (REA518, red) and counterstained with DRAQ5 (blue) as DNA stain.
View details
Overlay histogram showing flow cytometric analysis of CD49f knockout cells. Wild type (red) and knockout cells (blue) were stained with CD49f-PE, clone (REA518). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Overlay histogram showing flow cytometric analysis of CD49f knockout cells. Wild type (red) and knockout cells (blue) were stained with CD49f-PE, clone (REA518). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for CD49f Antibody, anti-human/mouse, REAfinity™

Overview

Clone REA518 recognizes the human and mouse CD49f antigen, a 120 kDa single-pass type I membrane protein, which is also known as integrin alpha-6 (ITGA6). Integrins are integral cell-surface proteins composed of an α chain and a β chain and are involved in cell adhesion as well as cell-surface mediated signaling. CD49f associates with integrin β1 chain (CD29) to form VLA-6 and with integrin β4 chain (CD104) to form the α6 β4 complex, which plays a critical structural role in the hemidesmosome and constitutes a receptor for laminin in epithelial cells. CD49f is furthermore expressed on T cells, monocytes, platelets, endothelial cells, trophoblasts of placenta, and spermatogonial stem cells.
Additional information: Clone REA518 displays negligible binding to Fc receptors.

Alternative names

ITGA6, CD49fB, VLA-6, VLA-6α

Detailed product information

Technical specifications

CloneREA518
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman, mouse, non-human primate, other
Cross-reactivityporcine, dog,
rhesus monkey (
Macaca mulatta
)
,
cynomolgus monkey (
Macaca fascicularis
)
,
olive baboon (
Papio anubis
)
,
chimpanzee (
Pan troglodytes
)
, capuchin monkey
AntigenCD49f
Alternative names of antigenITGA6, CD49fB, VLA-6, VLA-6α
Molecular mass of antigen [kDa]124, 120(separately shown for human and mouse/rat)
Distribution of antigenstem cells, epithelial cells, lymphocytes, megakaryocytes, monocytes, platelets, T cells, cancer stem cells, hematopoietic stem and progenitor cells, ES and iPS cells, thymocytes, brain, kidney, liver, lung, ovary, spleen
Entrez Gene ID3655, 16403
RRIDAB_2751828, AB_2802026, AB_2802009, AB_2802050, AB_2802044, AB_2751859

Resources for CD49f Antibody, anti-human/mouse, REAfinity™

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for CD49f Antibody, anti-human/mouse, REAfinity™

Publications

  1. Hynes, R. O. (2002) Integrins: bidirectional, allosteric signaling machines. Cell 110(6): 673-687
  2. Aumailley, M. et al. (1990) Antibody to integrin α 6 subunit specifically inhibits cell-binding to laminin fragment 8. Exp. Cell Res. 188(1): 55-60
  3. Hemler, M. E. et al. (1988) Multiple very late antigen (VLA) heterodimers on platelets. Evidence for distinct VLA-2, VLA-5 (fibronectin receptor), and VLA-6 structures. J. Biol. Chem. 263: 7660-7665
  4. Carter, W. G. et al. (1990) Distinct functions for integrins α 3 β 1 in focal adhesions and α 6 β 4/bullous pemphigoid antigen in a new stable anchoring contact (SAC) of keratinocytes: relation to hemidesmosomes. J. Cell Biol. 111: 3141-3154

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