Name: CD44 Antibody, anti-mouse
Availability: InStock

Flow cytometry

Conjugate:	PE-Vio 770
Recommended dilution:	1:10
Remark:	The antibody is suited for staining of formaldehyde-fixed cells.
Tested:	QC tested
Products used:	130-102-904, 130-102-377

MICS (MACSima Imaging Cyclic Staining)

Conjugate:	APC
Recommended dilution:	1:10
Fixation:	PFA
Tested:	during development
Products used:	130-102-563
Compatible applications:	IF, IHC

Extended validation for CD44 Antibody, anti-mouse

Specificity

Epitope competition

In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.

Other clones	Overlap in epitope recognition with IM7.8.1
IM7	++
REA664	++
Cells were incubated with an excess of purified unconjugated CD44 (IM7.8.1) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Specifications for CD44 Antibody, anti-mouse

Overview

Mouse CD44 is also known as Pgp-1, HCELL, HUTCH-I, HCAM, HERMES, and Ly-24. Clone IM7 reacts with murine CD44, an 80–95 kDa membrane glycoprotein. It is expressed by a variety of hematopoetic and non-hematopoetic cells. Different isoforms are generated by alternative splicing. Clone IM7 reacts with all isoforms and both allelic forms of CD44. CD44 functions as a receptor for hyaluronic acid (HA) and mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). CD44 expression is upregulated on naive T cells upon activation and high CD44 expression is maintained on memory T cells. Therefore, CD44 is often used to discriminate CD44

^hi^gh

antigen-experienced T cells from CD44

^lo^w

(CD62L

⁺

) naive T cells.

Alternative names

HERMES, Ly-24, Pgp-1, CD44s, EMCR III, H-CAM

Detailed product information

Technical specifications

Clone	IM7.8.1
Clonality	monoclonal
Isotype	rat IgG2bκ
Isotype control	Isotype Control Antibody, rat IgG2b
Host	rat
Type of antibody	Primary antibodies
Species	mouse, non-human primate, other
Cross-reactivity	rhesus monkey ( Macaca mulatta ) , cynomolgus monkey ( Macaca fascicularis ) , chimpanzee ( Pan troglodytes ) , baboon, human, pig, cow, horse, cat, dog
Antigen	CD44
Alternative names of antigen	HERMES, Ly-24, Pgp-1, CD44s, EMCR III, H-CAM
Molecular mass of antigen [kDa]	83
Distribution of antigen	endothelial cells, T cells, red blood cells, stem cells, leukocytes, lymphocytes, cancer stem cells, CNS cells, mesenchymal stem cells, plasma cells, ES and iPS cells, bone marrow, kidney, skeletal muscle, skin, lymphocytes, endothelial cells, T cells, red blood cells, stem cells, leukocytes, cancer stem cells, CNS cells, mesenchymal stem cells, plasma cells, ES and iPS cells, bone marrow, kidney, skeletal muscle, skin
Entrez Gene ID	12505
RRID	AB_2727594, AB_2819675, AB_2658178, AB_2658179, AB_2658180, AB_2658181, AB_2658183, AB_2658186, AB_2658187, AB_2658190, AB_2658191, AB_2727570

References for CD44 Antibody, anti-mouse

Publications

Ponta, H. et al. (2003) CD44: From adhesion molecules to signalling regulators. Nat. Rev. Mol. Cell Biol. 4(1): 33-45

Applications

Extended validation

Extended validation for CD44 Antibody, anti-mouse

Specificity

Epitope competition

In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.

Other clones	Overlap in epitope recognition with IM7.8.1
IM7	++
REA664	++
Cells were incubated with an excess of purified unconjugated CD44 (IM7.8.1) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Specifications

Specifications for CD44 Antibody, anti-mouse

Overview

^hi^gh

antigen-experienced T cells from CD44

^lo^w

(CD62L

⁺

) naive T cells.

Alternative names

HERMES, Ly-24, Pgp-1, CD44s, EMCR III, H-CAM

Detailed product information

Technical specifications

Clone	IM7.8.1
Clonality	monoclonal
Isotype	rat IgG2bκ
Isotype control	Isotype Control Antibody, rat IgG2b
Host	rat
Type of antibody	Primary antibodies
Species	mouse, non-human primate, other
Cross-reactivity	rhesus monkey ( Macaca mulatta ) , cynomolgus monkey ( Macaca fascicularis ) , chimpanzee ( Pan troglodytes ) , baboon, human, pig, cow, horse, cat, dog
Antigen	CD44
Alternative names of antigen	HERMES, Ly-24, Pgp-1, CD44s, EMCR III, H-CAM
Molecular mass of antigen [kDa]	83
Distribution of antigen	endothelial cells, T cells, red blood cells, stem cells, leukocytes, lymphocytes, cancer stem cells, CNS cells, mesenchymal stem cells, plasma cells, ES and iPS cells, bone marrow, kidney, skeletal muscle, skin, lymphocytes, endothelial cells, T cells, red blood cells, stem cells, leukocytes, cancer stem cells, CNS cells, mesenchymal stem cells, plasma cells, ES and iPS cells, bone marrow, kidney, skeletal muscle, skin
Entrez Gene ID	12505
RRID	AB_2727594, AB_2819675, AB_2658178, AB_2658179, AB_2658180, AB_2658181, AB_2658183, AB_2658186, AB_2658187, AB_2658190, AB_2658191, AB_2727570

References

References for CD44 Antibody, anti-mouse

Publications

Ponta, H. et al. (2003) CD44: From adhesion molecules to signalling regulators. Nat. Rev. Mol. Cell Biol. 4(1): 33-45

CD44 Antibody, anti-mouse

CD44 Antibody, anti-mouse

Flow cytometry

MICS (MACSima Imaging Cyclic Staining)

Extended validation for CD44 Antibody, anti-mouse

Specificity

Specifications for CD44 Antibody, anti-mouse

Overview

Alternative names

Detailed product information

Technical specifications

References for CD44 Antibody, anti-mouse

Publications

Applications

Extended validation

Extended validation for CD44 Antibody, anti-mouse

Specificity

Specifications

Specifications for CD44 Antibody, anti-mouse

Overview

Alternative names

Detailed product information

Technical specifications

References

References for CD44 Antibody, anti-mouse

Publications

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CD44 Antibody, anti-mouse

CD44 Antibody, anti-mouse

Flow cytometry

MICS (MACSima Imaging Cyclic Staining)

Extended validation for CD44 Antibody, anti-mouse

Specificity

Specifications for CD44 Antibody, anti-mouse

Overview

Alternative names

Detailed product information

Technical specifications

References for CD44 Antibody, anti-mouse

Publications

Applications

Extended validation

Extended validation for CD44 Antibody, anti-mouse

Specificity

Specifications

Specifications for CD44 Antibody, anti-mouse

Overview

Alternative names

Detailed product information

Technical specifications

References

References for CD44 Antibody, anti-mouse

Publications

Shop

Careers

About Miltenyi Biotec